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Comparison of systemic inflammatory and hematology parameters in normal C57BI/6 and genetically diabetic db/db mice during local wound repair
Michael J. Parnham
Puni tekst: engleski, pdf (163 KB)
Šitum, K., Bokulić, A., Ivetić-Tkalčević, V., Parnham, M.J., Čužić, S., Durić, K., Glojnarić, I., Ševeljević-Jaran, D., Brajša, K. (2007). Comparison of systemic inflammatory and hematology parameters in normal C57BI/6 and genetically diabetic db/db mice during local wound repair. Biochemia medica, 17(1), 85-93. Preuzeto s http://hrcak.srce.hr/12916
Puni tekst: hrvatski, pdf (163 KB)
Šitum, K., Bokulić, A., Ivetić-Tkalčević, V., Parnham, M.J., Čužić, S., Durić, K., Glojnarić, I., Ševeljević-Jaran, D., Brajša, K. (2007). Usporedba sustavnih upalnih i hematoloških parametara kod zdravih C57B1/6 i genetski dijabetičnih db/db miševa tijekom cijeljenja lokalne rane. Biochemia medica, 17(1), 85-93. Preuzeto s http://hrcak.srce.hr/12916
Introduction: Inflammation is the initial host response to injury. It is not only localized to the wound site but also causes systemic changes, including a variety of physiological and biochemical changes collectively called the acute phase response. These changes continue during the resolution of inflammation and the wound healing process. In this study we compared serum amyloid A protein (SAA), hematological parameters (total white blood cell count, neutrophil and lymphocyte percentage) and interferon-gamma (IFN-γ) concentrations in serum during healing of non-occluded, excisional, full-thickness dermal wounds in genetically diabetic db/db mice and non-diabetic C57Bl/6 littermates.
Materials and Methods: Area of a punch biopsy (8 mm in diameter) wound in each mouse was analyzed by computer-assisted planimetry. On days 3,6, 9 and 13 after wounding, SAA and IFN-γ were measured in plasma by ELISA assays and hematological parameters in whole blood by SysmexSF 3000 automatic hematology analyzer.
Results: A delay in the closure of wounds in db/db in comparison to normal mice was clearly seen on days 6 and 9. Total white blood cell count was significantly higher on days 9 and 13 in db/db mice. Differential neutrophil counts were higher and lymphocyte counts lower in db/db mice in comparison to C57BL/6 mice throughout the wound repair period. Peak SAA concentrations were seen on day 3 in C57Bl/6 and db/db mice (368.7 mg/L and 173.5 mg/L, respectively), but tended to be lower in db/db mice. IFN-γ levels were significantly higher (P < 0.05) on days 9 and 13 in db/db (75.3 pg/mL and 89.9 pg/mL, respectively) in comparison to those in C57Bl/6 mice (66.6 pg/mL and 57.2 pg/mL, respectively).
Conclusion. The local tissue regeneration process in mice after local skin injury causes systemic changes in peripheral blood. Determination of neither SAA nor IFN-γ concentrations could be used to monitor wound healing dynamics at these time points.
db/db mice; serum amyloid A protein; interferon-gamma; inflammation
Hrčak ID: 12916
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