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Surgical Technique in the Rat Model of Kidney Transplantation
Josip Španjol
Tanja Čelić
Tomislav Jakljević
Aldo Ivančić
Dean Markić
Sažetak
Today successful kidney transplantation procedures, techniques and immunosupression protocols are a consequence
of extensive research on animal models. During every transplantation surgery there are two crucial points for the success
of the entire procedure: vascular (arterial end venous) and ureteral or ureterovesical anastomosis. Renal artery and vein
of the donor kidney can be anastomosed end-to-side to the abdominal aorta and vena cava of the recipient (heterotopic
transplantation), or end-to-end to the remains of renal artery and vain of the recipient (orthotopic transplantation) after
nepherctomy. The ureter can be anastomosed also end-to-en or we can connect it directly to the urinary bladder (ureterocystoneostomy).
The aim of this study was to elucidate which technique has better results according to: animal survival,
reperfusion and perfusion of the transplanted kidney, elimination of the urine from the transplanted kidney and procedure
costs. The study included 240 (120 donors and 120 recipients) male Wistar rats (3 months old; weight 250–300 g
Our results are clearly showing that the end-to-end vascular anastomosis, and Paquins ureterovesical anastomosis have
better results in transplanted rat kidneys survival and urine drainage compared to end-to-side vascular anastomosis
and end-to-end ureteral anastomosis. Based on our experience we can conclude that described methods of end-to-end vascular
anastomosis and Paquins ureterovesical anastomosis are less technically demanding and have a shorter learning
curve. Therefore, we can recommend the use of described methods in kidney transplantation related researches.
Ključne riječi
kidney transplantation; rat model; urine drainage; vascular anastomosis
Hrčak ID:
72236
URI
Datum izdavanja:
25.9.2011.
Posjeta: 1.030 *