Izvorni znanstveni članak

Digital thermography in analysis of temperature changes in Pelophylax ridibundus frog

DOMAGOJ ĐIKIĆ ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
DARKO KOLARIĆ ; Ruđer Bošković Institute, Centre for Informatics and Computing, Zagreb, Croatia
VESNA BENKOVIĆ ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
ANICA HORVAT-KNEŽEVIĆ ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
KATARINA ŠKOLNIK GADANAC ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
ZORAN TADIĆ ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia
NADA ORŠOLIĆ ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, 10000 Zagreb, Croatia

Sažetak

Background and Purpose: Physiological field of metabolism manipulation tries to elucidate how tissues recuperate after ischemic reperfusion changes, how signal molecules coordinate metabolic pathways and what physiological changes are to be expected in induced artificial hypometabolism or suspended animation in biomedicine. Evolutionary developed mechanisms of lowered metabolism (torpor, hibernation and aestivation) followed
by arousals to normal metabolic/thermoregulatory states present
perfect models for such studies. In the light of the vast current interest inmanipulating metabolism, natural behavior and adaptations of frogs, makes them among other organisms, an appropriate standard model animal for such studies. The exact measurements of thermal changes of frog’s body temperature correlated with ambient temperature (Ta) changes are essential.

Materials and Methods: Male frogs Pelophylax ridibundus (Eurasian
Marsh Frog) were kept for 30 days at Ta=8°C (artificial hibernation) and then exposed to Ta = 23°C (artificial arousal). The dynamics of body temperature change over 146 minutes was analyzed with IR camera NEC Thermo tracer TH7102WL and ThermoWEB measuring system.

Results and Conclusions: Use of thermography allowed real time thermal measurement of changes in body temperature in frogs in a noninvasive manner. Previous attempts at thermography in hibernating frogs have not been reported, perhaps because of the lack of precision of earlier instruments. New generation cameras have the accuracy and software support to discriminate subtle differences in temperature of different body regions of analyzed frogs and the surrounding environment, as documented in this
study.

Ključne riječi

thermography; thermal scanning; cold exposure; frog; hibernation; hypometabolism

Hrčak ID:

76956

URI

https://hrcak.srce.hr/76956

Datum izdavanja:

15.11.2011.

Posjeta: 1.578 *