Izvorni znanstveni članak
Morphological alterations of T24 cells on flat and nanotubular TiO2 surfaces
Roghayeh Imani
; Faculty of Electrical Engineering University of Ljubljana, Ljubljana, Slovenia
Doron Kabaso
; Laboratory of Neuroendocrinology-Molecular Cell Physiology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Mateja Erdani Kreft
; Institute of Cell Biology, Faculty of Medicine, University of Ljubljana, Ljubljana, Slovenia
Ekaterina Gongadze
; Faculty of Electrical Engineering University of Ljubljana, Ljubljana, Slovenia
Samo Penič
; Faculty of Electrical Engineering University of Ljubljana, Ljubljana, Slovenia
Kristina Eleršič
; J. Stefan Institute, Ljubljana, Slovenia
Andrej Kos
; Faculty of Electrical Engineering University of Ljubljana, Ljubljana, Slovenia
Peter Veranič
; Institute of Cell Biology, Faculty Medicine, University of Ljubljana, of Ljubljana, Slovenia
Robert Zorec
; Celica Biomedical Center Ljubljana, Slovenia
Aleš Iglič
; Faculty of Electrical Engineering University of Ljubljana, Ljubljana, Slovenia
Sažetak
Aim To investigate morphological alterations of malignant
cancer cells (T24) of urothelial origin seeded on flat titanium
(Ti) and nanotubular titanium dioxide (TiO2) nanostructures.
Methods Using anodization method, TiO2 surfaces composed
of vertically aligned nanotubes of 50-100 nm diameters
were produced. The flat Ti surface was used as a reference.
The alteration in the morphology of cancer cells was
evaluated using scanning electron microscopy (SEM). A
computational model, based on the theory of membrane
elasticity, was constructed to shed light on the biophysical
mechanisms responsible for the observed changes in the
contact area of adhesion.
Results Large diameter TiO2 nanotubes exhibited a significantly
smaller contact area of adhesion (P < 0.0001) and
had more membrane protrusions (eg, microvilli and intercellular
membrane nanotubes) than on flat Ti surface. Numerical
membrane dynamics simulations revealed that the
low adhesion energy per unit area would hinder the cell
spreading on the large diameter TiO2 nanotubular surface,
thus explaining the small contact area.
Conclusion The reduction in the cell contact area in the
case of large diameter TiO2 nanotube surface, which does
not enable formation of the large enough number of the focal
adhesion points, prevents spreading of urothelial cells.
Ključne riječi
Hrčak ID:
95004
URI
Datum izdavanja:
15.12.2012.
Posjeta: 955 *