Izvorni znanstveni članak
https://doi.org/10.15177/seefor.13-01
Lead Tolerance and Accumulation in White Poplar Cultivated In Vitro
Branislav Kovačević
orcid.org/0000-0002-9125-0659
; University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia
Dragana Miladinović
orcid.org/0000-0001-9555-9162
; Institute of Field and Vegetable Crops, Novi Sad, Serbia
Saša Orlović
; University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia
Marina Katanić
orcid.org/0000-0003-2993-1637
; University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia
Marko Kebert
; University of Novi Sad, Institute of Lowland Forestry and Environment, Novi Sad, Serbia
Jovana Kovinčić
; University of Novi Sad, Faculty of Agriculture, Novi Sad, Republic of Serbia
Sažetak
Background and Purpose: This paper analyses the lead tolerance and accumulation in white poplar genotypes in vitro, in order to optimize genotype evaluation and other procedures in their implementation in phytoremediation projects and landscaping in areas endangered by lead accumulation.
Material and Methods: The lead tolerance and accumulation of five white poplar genotypes after 35 days in vitro cultivation on media supplemented with lead was examined. The following Pb(NO3)2 concentrations were used: 0, 10-6, 10-5, 10-4 and 10-3 M. Tolerance analysis (described by tolerance indices) was based on morphological parameters, biomass accumulation and the content of photosynthetic pigments, while lead accumulation was described by shoot lead accumulation and shoot lead content.
Results and Conclusions: The chosen lead concentrations appeared not to be lethal. Moreover, the obtained results showed that the tested lead concentrations had a positive effect on: number of formed roots, shoot moisture content and shoot height. The best differentiation among the examined genotypes was gained by the tolerance index based on the shoot height on 10-4 M Pb(NO3)2. The shoot lead accumulation and shoot lead content significantly increased on 10-4 and 10-3 M Pb(NO3)2 media. Thus, the concentration of 10-4 M Pb(NO3)2 is recommended for further research. Two examined genotypes of horticultural value (LCM and LBM) achieved a significantly higher lead shoot content compared to the wide spread genotype “Villafranca” (almost 200% and 125% higher, respectively).
Ključne riječi
Populus alba; tissue culture; phytoextraction
Hrčak ID:
106306
URI
Datum izdavanja:
28.6.2013.
Posjeta: 1.036 *