Izvorni znanstveni članak
Antibiotic susceptibility and antimicrobial activity of autochthonous starter cultures as safety parameters for fresh cheese production
Andreja Leboš Pavunc
Blaženka Kos
Jasna Beganović
orcid.org/0000-0003-1374-1097
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for Antibiotics, Enzymes, Probiotics and Starter Cultures Technology, Pierottijeva 6, 10000 Zagreb, Croatia
Ksenija Uroić
Dora Bučan
Jagoda Šušković
Sažetak
The antibiotic susceptibility and antimicrobial activity, as food safety parameters important for application of autochthonous lactic acid bacteria (LAB), that previously satisfied technological criteria for functional starter cultures in fresh cheese production were examined. Soluble whole cell protein patterns of autochthonous LAB strains from fresh cheese, obtained by SDS-PAGE, revealed the presence of two predominant strains, which were identified as Lactobacillus fermentum A8 and Enterococcus faecium A7. These strains were not resistant and shown susceptibility to antibiotics: ampicillin, bacitracin, penicillin G, azithromycin, chloramphenicol, clarithromycin, clindamycin, spiramycin, tetracycline, streptomycin, neomycin, gentamicin, erythromycin, rifampicin and novobiocin. Lb. fermentum A8 strain displayed phenotypic resistance to vancomycin, but this resistance is intrinsic, not transferable and it is acceptable from the safety aspect. The capacity of Lb. fermentum A8 and Ec. faecium A7 to inhibit growth of test-microorganisms Listeria monocytogenes ATCC 11911, Escherichia coli 3014, Salmonella enterica serovar Typhimurium FP1 and Staphylococcus aureus 3048, was also analysed. According to obtained results, Lb. fermentum A8 and Ec. faecium A7 are safe from the aspect of spreading antibiotic resistance and could be useful as bioprotective cultures that inhibit common bacterial food contaminants, including L. monocytogenes.
Ključne riječi
antimicrobial activity; antibiotic susceptibility; autochthonous starter cultures
Hrčak ID:
111096
URI
Datum izdavanja:
25.11.2013.
Posjeta: 2.386 *