Izvorni znanstveni članak

https://doi.org/10.2478/acph-2014-0014

HPLC method with monolithic column for simultaneous determination of irbesartan and hydrochlorothiazide in tablets

AMER M. ALANAZI ; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457 Riyadh, 11451, Kingdom of Saudi Arabia
ALI S. ABDELHAMEED ; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457 Riyadh, 11451, Kingdom of Saudi Arabia
NASR Y. KHALIL ; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457 Riyadh, 11451, Kingdom of Saudi Arabia
AZMAT A. KHAN ; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457 Riyadh, 11451, Kingdom of Saudi Arabia
IBRAHIM A. DARWISH ; Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, P.O. Box 2457 Riyadh, 11451, Kingdom of Saudi Arabia

Sažetak

A simple, sensitive and accurate HPLC method with high throughput has been developed and validated for the simultaneous determination of irbesartan (IRB) and hydrochlorothiazide (HCT) in combined pharmaceutical dosage forms. The proposed method employed, for the first time, a monolithic column in the analysis. Optimal chromatographic separation of the analytes was achieved on Chromolith® Performance RP-18e column using a mobile phase consisting of phosphate buffer (pH 4):acetonitrile (50:50, V/V) pumped isocratically at a flow rate of 1.0 mL min–1. The eluted analytes were monitored with a UV detector set at 270 nm. Under the optimum chromatographic conditions, linear relationship with a good correlation coefficient (R ≥ 0.9997) was found between the peak area and the corresponding concentrations of both IRB and HCT in the ranges of 10–200 and 1–20 ng mL–1. The limits of detection were 2.34 and 0.03 ng mL-1 for IRB and HCT, respectively. The intra- and inter-assay precisions were satisfactory as the RSD values did not exceed 3 %. The accuracy of the proposed method was > 97 %. The proposed method had high throughput as the analysis involved a simple procedure and a very short run-time of 3 min. The results demonstrated that the method is applicable in the quality control of combined pharmaceutical tablets containing IRB and HCT.

Ključne riječi

irbesartan; hydrochlorothiazide; HPLC-UV; monolithic column

Hrčak ID:

114598

URI

https://hrcak.srce.hr/114598

Datum izdavanja:

30.6.2014.

Posjeta: 1.760 *