Izvorni znanstveni članak
Genetic Polymorphisms of 15 STR Loci within Turkish Student Population Living in Sarajevo, Bosnia and Herzegovina
Serkan Dogan
; International Burch University, Department of Genetics and Bioengineering, Sarajevo, Bosnia and Herzegovina
Lejla Kovačević
; University of Sarajevo, Faculty of Pharmacy, Sarajevo, Bosnia and Herzegovina
Damir Marjanović
; University of Sarajevo, Institute for Genetic Engineering and Biotechnology, Sarajevo, Bosnia and Herzegovina
Sažetak
Allele frequencies of 15 STRs included in the PowerPlex 16 System (D3S1358, TH01, D21S11, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSF1PO, Penta D, VWA, D8S1179, TPOX and FGA) were calculated from the referent sample of 100 unrelated individuals of both sexes from Turkish student population living in Sarajevo, Bosnia and Herzegovina. Buccal swab, as a source of DNA, was collected from the volunteers from whom the informed consent form was obtained. DNA extraction was performed using QIAamp DNA Micro kit by Qiagen. DNA template ranging from 0.5 to 2 ng was used to amplify 15 STR loci by PCR multiplex amplification which was performed by using the PowerPlex 16 kit (Promega Corp., Madison, WI, USA) according to the manufacturer’s protocol. The amplifications were carried out in a PE Gene Amp PCR System thermal cycler (Applied Biosystems) and capillary electrophoresis was car- ried out in an ABI PRISM 310 Genetic Analyzer (Applied Biosystems) in accordance with the manufacturer’s recommen- dations. The frequency of each locus was calculated from the numbers of each observed genotype. Deviation from Hardy- -Weinberg equilibrium and observed heterozygosity were calculated. Data were analyzed by using Microsoft Excel work- book template – Powerstats V12 and the power of discrimination (PD), power of exclusion (PE), as well as other popula- tion genetic indices for the 15 STR loci were calculated. Obtained results contribute to existing Turkish DNA database, as well as insight of differences and similarities in comparison to population of Bosnia and Herzegovina. In addition, 13 autosomal STR loci frequencies (D3S1358, TH01, D21S1 1, D18S51, Penta E, D5S818, D13S317, D7S820, D16S539, CSFIPO, Penta D, VWA, D8S1 179, TPOX, and FGA) were studied in 15 different worldwide populations (Turkish, Bosnian, Croatian, Serbian, Montenegrin, Macedonian, Albanian, Kosovan, Greek, Russian, Japanese, Korean, Lithua- nian, Iraqi, Belarusian). For the proof of corresponding data, two different Turkish population STR data obtained from previously published articles were compared with our data and this showed that our data correspond to these 2 previ- ously published data. Further, STR allele frequency data for 13 loci for each population were obtained from previous sci- entific articles and the allele frequencies and genetic diversity among the 15 sample populations were compared. In addi- tion, even though the populations are from different nationalities, the STR data are similar among the geographically close populations. The phylogenetic tree established among worldwide populations and genetic distance values show a great affinity among the 15 populations. Our data is useful for anthropological and further comparative genetic studies of populations.
Ključne riječi
STR loci; allele frequencies; DNA typing; Turkish population data; polymorphisms; phylogenetic tree
Hrčak ID:
118389
URI
Datum izdavanja:
30.12.2013.
Posjeta: 1.019 *