Skoči na glavni sadržaj

Izvorni znanstveni članak

First evidence of the presence of Multixenobiotic Resistance Mechanism activity in freshwater invasive species, signal crayfish Pacifastacus leniusculus (Dana, 1852)

ANA BIELEN ; Laboratory for Biology and Microbial Genetics, Department for Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
TOMISLAV VLADUŠIĆ ; Laboratory for Biology and Microbial Genetics, Department for Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
NIKOLINA KUHARIĆ ; Department of Zoology, Division of Biology, Faculty of Science, Rooseveltov trg 6, 10000 Zagreb, Croatia
SANDRA HUDINA ; Department of Zoology, Division of Biology, Faculty of Science, Rooseveltov trg 6, 10000 Zagreb, Croatia
LIDIJA ŠVER ; Laboratory for Biology and Microbial Genetics, Department for Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia
SAŠA LIKIĆ ; Department of Botany, Division of Biology, Faculty of Science, Marulićev trg 9a, 10000 Zagreb, Croatia
IVANA BOŠNJAK ; Laboratory for Biology and Microbial Genetics, Department for Biochemical Engineering, Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, 10000 Zagreb, Croatia


Puni tekst: engleski pdf 598 Kb

str. 267-273

preuzimanja: 598

citiraj


Sažetak

Background and Purpose: The signal crayfish Pacifastacus leniusculus
(Dana, 1852) is one of the most successful invasive species of crayfish in
European freshwaters, an extremely diverse though endangered group of
ecosystems. The main goal of this study was to functionally characterize
multixenobiotic resistance (MXR) mechanism defense activity in P. leniusculus tissues for the first time. MXR mechanism protects the cell from a wide variety of toxic compounds, and it is mediated by the transport activity of ATP-binding cassette (ABC) proteins.

Materials and Methods: MXR transporter activity dye assay was performed
by using fluorescent model substrate rhodamine B (RB) in combination
with inhibitors of MXR efflux pumps: MK571 and Verapamil, known to
inhibit multidrug resistance-associated proteins (MRP) and P-glycoprotein
(P-gp), respectively. In this assay, the increase in intracellular fluorescence of the substrate dye, indicates inhibition of MXR efflux protein pumps. The assay was performed in three different tissues (gills, hepatopancreas, tail muscle). Additionally, tissues were exposed to selected heavy metals – mercury (HgCl2) and zinc (ZnCl2), known to occur in open freshwaters as pollutants.

Results: Optimal time for RB accumulation in gills and hepatopancreas
was determined to be 30 minutes. RB efflux in gills was inhibited by MK571
and in hepatopancreas by Verapamil, suggesting that multidrug resistanceassociated proteins are dominant in gills of P. leniusculus, and P-glycoprotein in hepatopancreas. Finally, inhibitory effect of mercury (HgCl2: 10 and 20 μM) and zinc (ZnCl2: 5–20 μM) on multixenobiotic resistance mechanism activity in gills, and only mercury in hepatopancreas, was detected.

Conclusions: The results for the first time demonstrate the presence of
multixenobiotic resistance mechanism efflux activity as an important tissue
specific defense mechanism in P. leniusculus and provide the basis for future molecular and toxicological studies of this invasive and adaptable species.

Ključne riječi

heavy metals; multixenobiotic resistance mechanism; Pacifastacus leniusculus; signal crayfish

Hrčak ID:

135148

URI

https://hrcak.srce.hr/135148

Datum izdavanja:

30.9.2014.

Posjeta: 1.211 *