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Distribution of Elders (Sambucus nigra L. and S. racemosa L.) Infected with Cherry Leaf Roll Virus in Yugoslavia

Julijana Grbelja ; BiH


Puni tekst: hrvatski pdf 1.984 Kb

str. 29-36

preuzimanja: 268

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Sažetak

Virus infected elders were found for the first time in Yugoslavia by Plavšić-Banjac and Miličić (1968). Afterwards Štefanac (1968) established that these plants were infected with cherry leaf roll virus (CLRV). By comparing an elder isolate (Bz S) with CLRV isolates obtained from cherry and rhubarb, Šefanac (1969) found that the elder isolate was serologically different from them because the isolates provoked spurs in agar-gel diffusion tests. During these investigations only two localities of infected elders were found in Yugoslavia.
In this work an attempt was made to establish the distribution of infected elders in this country more precisely. Elders suffering from virus diseases were detected in some new localities in Sarajevo, they abounded on the citadel hillsides in Ljubljana, in many places on Zagrebačka gora, and also at Slunj and Labin (Fig. 1). The disease was also present and widely spread in Sambucus racemosa on Zagrebačka gora.
Six virus isolates from Sambucus nigra, taken from various localities, and one from S. racemosa were examined in detail. Seedlings of both elder species infected with these isolates first developed vein-clearing (Fig. 4A), then chlorotic spots, rings and lines on leaves. Chenopodium quinoa, C. amaranticolor and C. murale produced chlorotic or necrotic local lesions on inoculated leaves and subseqently the plant apices withered and eventually the whole plants died (Fig. 3). Nicotiana clevelandii, N. langsdorffii, N. maritima, N. megalosiphon, N. rustica, N. sylvestris, N. tabacum (Hicks resistant, Samsun and White Burley) and N. sp. (Wisconsin 38) reacted with concentric necrotic rings (Fig. 2). After these investigations it was clear that seven examined isolates produce the same symptoms, i.e. that these isolates belong to the same virus. The symptoms on test plants were characteristic of CLRV.
Thermal inactivation point of S. nigra and S. racemosa isolates was estimated several times. The squashed sap from virus infected plants was infective at 52 °C, but not at 55 °C. The dilution end point of the isolates was between 1 :1000 and 1 :10000, and longevity in vitro from three to five days at room temperature. The virus suspension stored in refrigerator at —15 °C was infective for more than sixteen days. Leaves of infected Chenopodium quinoa dried in desiccator remained infective at + 2 °C even after a year.
The Bz S isolate of CLRV was purified and an antiserum prepared with homologous titre of 1/256. In agar-gel diffusion tests all isolates showed positive reaction with this serum. Their precipitin lines were connected without spurs (Fig. 4B) and showed that all S. nigra and S. racemosa isolates were serologically identical. They were identical also with the BzS isolate examined by Stefanac (1969) (Fig. 4B). The results of serological reactions also prove that the investigated isolates belong to the elder strain of CLRV.

Ključne riječi

Hrčak ID:

157125

URI

https://hrcak.srce.hr/157125

Datum izdavanja:

31.12.1972.

Podaci na drugim jezicima: hrvatski

Posjeta: 735 *