Izvorni znanstveni članak
In vitro and in planta Agrobacterium tumefaciens - mediated Transformation of Arabidopsis thaliana
Tatjana Bakran-Petricioli
; Zoologijski zavod PMF-a, Sveučilište u Zagrebu
Sibila Jelaska
; Zavod za molekularnu biologiju PMF-a, Sveučilište u Zagrebu
Sažetak
Cell transformation of Arabidopsis thaliana (L.) Heynh. with Agrobacterium tumefaciens (LBA4404 strain) was performed in two ways: in vitro - by the transformation of root and stem explants - and inplanta - by the transformation of whole plants in the greenhouse. Agrobacterium contained the binary plasmid pBI121 or its constructs with the fragments of carrot DC-8 promoter. The results show that transformation efficiency in vitro is influenced by the age and type of explant. It was not possible to induce transgenic calli on explants of 5 week old Arabidopsis roots, although it was possible on explants of 3 week old roots. Although green calli grew on Arabidopsis stem explants, transgenic shoots did not develop. When using transformation in vitro, from seed to mature primary transformant it took 15 to 16 weeks and for in planta transformation it took 19 to 20 weeks.
Ključne riječi
Hrčak ID:
160460
URI
Datum izdavanja:
31.12.1997.
Posjeta: 1.007 *