Izvorni znanstveni članak

The interaction of jasmonic acid, sucrose and light is reflected in photosynthetic pigment metabolism in potatoes grown in vitro

Maja Kovač ; National Institute of Biology, Ljubljana, Slovenia
Maja Ravnikar ; National Institute of Biology, Ljubljana, Slovenia

Sažetak

We previously demonstrated that sucrose and jasmonic acid (JA) interact duringin photosynthetic in the regulation of development and pigment metabolism of the potato (Solanum tuberosum L cv. Sante) grown in vitro. In this study, the research was extended to the effect of light irradiance and quality on sucrose and JA mediated changes. Potato plantlets grown from stem node culture were grown on a medium supplemented with 30 mM or 90 mM sucrose and 0.1, 1, or 10 μM JA at 55-70 μmol m-2 s-1 (Osram Cool White lights) or at 80-100 μmol m-2 s-1 (Osram Fluora lights). Higher amounts of chlorophyll were detected in plants grown at lower irradiance while there was no significant difference in carotenoid levels. Sucrose and JA both enhanced root growth and inhibited photosynthetic pigment accumulation. Most effective was 1 μM JA at higher sucrose concentrations. In these growth conditions the plantlets were hyperhydric and had the most developed root system. The present study demonstrates that the growth of plants in vitro and the photosynthetic pigment metabolism are highly dependent on the interaction of a number of environmental factors such as sucrose and plant growth regulators in the medium, and light irradiance and quality. This result supports our previous findings that the reduction of photosynthetic pigment accumulation in cv. Sante potato plantlets induced by JA could be mainly the consequence of enhanced import of sucrose from the medium.

Ključne riječi

Solanum tuberosum; tissue culture; jasmonic acid; sucrose uptake; irradiation, photosynthetic pigments

Hrčak ID:

160590

URI

https://hrcak.srce.hr/160590

Datum izdavanja:

31.12.1999.

Posjeta: 706 *