Izvorni znanstveni članak

https://doi.org/10.1515/acph-2016-0040

Development and validation of HPLC and CE methods for simultaneous determination of amlodipine and atorvastatin in the presence of their acidic degradation products in tablets

SAID A. HASSAN ; Faculty of Pharmacy, Department of Analytical Chemistry, 11562, Cairo, Egypt
EMAN S. ELZANFALY ; Faculty of Pharmacy, Department of Analytical Chemistry, 11562, Cairo, Egypt
MAISSA Y. SALEM ; Faculty of Pharmacy, Department of Analytical Chemistry, 11562, Cairo, Egypt
BADR A. EL-ZEANY ; Faculty of Pharmacy, Department of Analytical Chemistry, 11562, Cairo, Egypt

Sažetak

Two methods were developed for separation and quantitation of amlodipine (AML) and atorvastatin (ATV) in the presence of their acidic degradation products. The first method was a simple isocratic RP-HPLC method while the second was capillary electrophoresis (CE). Degradation products were obtained by acidic hydrolysis of the two drugs and their structures were elucidated for the first time by IR and MS spectra. Degradation products did not interfere with the determination of either drug and the assays were therefore stability-indicating. The linearity of the proposed methods was established over the ranges 1–50 μg mL–1 for AML and ATV in the HPLC method and in the range of 3–50 and 4–50 μg mL-1 for AML and ATV, respectively, in the CE method. The proposed methods were validated according to ICH guidelines. The methods were successfully applied to estimation of AML and ATV in bulk powder and in pharmaceutical dosage forms.

Ključne riječi

atorvastatin; amlodipine; capillary zone electrophoresis; HPLC; stability; acidic

Hrčak ID:

161221

URI

https://hrcak.srce.hr/161221

Datum izdavanja:

31.12.2016.

Posjeta: 1.541 *