Poljoprivreda, Vol. 13 No. 1, 2007.
Izvorni znanstveni članak
EFFECT OF FEED PROCESSING ON VOLATILE FATTY ACID PRODUCTION RATES MEASURED WITH 13C-ACETATE IN GRAZING LACTATING DAIRY COWS
R. Tothi
H.Z.H. Taweel
S. Tamminga
Sažetak
The effect of processed cereal grain supplementation on volatile fatty acid (VFA) production rates of grazing, lactating Holstein-Friesian cows were measured in a 5x5 Latin square experiment. The experimental treatments were as follows: control (only grazing, no supplement addition, NS), pelleted barley (PB), pelleted maize (PM), toasted and subsequently pelleted barley (TPB), and toasted and subsequently pelleted maize (TPM) as supplements. An isotope dilution technique using stable isotope of carbon (13C) as an internal marker was employed for the estimation of VFA production. At the beginning of a 3-hour long allowed grazing time, 100 mg of 99% enriched 13C2 Na-acetate were introduced in the rumen and repeated after grazing with 50 mg isotope, after which the cows were starved for 6 hours until evening milking. During grazing disappearance rate (kdis) and production rate (kprod) of acetate, propionate and butyrate were significantly higher (P≤0.05) in supplemented than in NS cows. Moreover the effect of barley grain and pelleting treatment was higher than the effect of maize grain and toasting. During starvation significantly higher (P≤0.05) kdis and kprod of VFAs were observed in PM and TPM treatments. Total VFA production for the experimental period (grazing + starvation) were 49.5, 78.7, 69.9, 88.5, 80.8 mol/day for NS, PB, TPB, PM and TPM, respectively. The higher VFA productions measured in supplemented animals emphasis the extensive digestion that occurs in the rumen after feeding processed grains. In methodological terms, 13C2 Na-acetate labelling appears to be a useful means for examining the VFA acetate production in ruminants.
Ključne riječi
dairy; grazing; supplementation; VFA production; stable isotope
Hrčak ID:
16068
URI
Datum izdavanja:
29.6.2007.
Posjeta: 1.746 *