Acta Pharmaceutica, Vol. 67 No. 2, 2017.
Izvorni znanstveni članak
https://doi.org/10.1515/acph-2017-0013
Comparing monolithic and fused core HPLC columns for fast chromatographic analysis of fat soluble vitamins
SAID EL KURDI
; Chemistry Department, Faculty of Science, Islamic University, Gaza, Palestine
DINA ABU MUAILEQ
; 2 Pharmaceutical Chemistry Department, Faculty of Pharmac, Al-Azhar University-Gaza, Gaza, Palestine
HASSAN A. ALHAZMI
; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Jazan University, Jazan, Saudi Arabia
MOHAMMED AL BRATTY
; Pharmaceutical Chemistry Department, Faculty of Pharmacy, Jazan University, Jazan, Saudi Arabia
SAMI EL DEEB
; Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, D-38106 Braunschweig, Germany
Sažetak
HPLC stationary phases of monolithic and fused core type can be used to achieve fast chromatographic separation as an alternative to UPLC. In this study, monolithic and fused core stationary phases are compared for fast separation of four fat-soluble vitamins. Three new methods on the first and second generation monolithic silica RP-18e columns and a fused core pentafluoro-phenyl propyl column were developed. Application of three fused core columns offered comparable separations of retinyl palmitate, DL-α-tocopheryl acetate, cholecalciferol and menadione in terms of elution speed and separation efficiency. Separation was achieved in approx. 5 min with good resolution (Rs >5) and precision (RSD ≤ 0.6 %). Monolithic columns showed, however, a higher number of theoretical plates, better precision and lower column backpressure than the fused core column. The three developed methods were successfully applied to separate and quantitate fat-soluble vitamins in commercial products.
Ključne riječi
monolithic silica column; fused core column; HPLC; fat-soluble vitamins; analytical performance
Hrčak ID:
172756
URI
Datum izdavanja:
30.6.2017.
Posjeta: 1.537 *