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Periodicum biologorum, Vol.118 No.4 Ožujak 2017.

Izvorni znanstveni članak
DOI: 10.18054/pb.v118i4.3856

Contribution to Globularia phylogeny based on nuclear ribosomal spacer and two chloroplast DNA regions

Kroata Hazler Pilepić
Maja Friščić
Ahmet Duran
Semir Maslo
Rade Garić
Sara Čuljak
Kristina Šutalo

Puni tekst: engleski, PDF (2 MB) str. 417-424 preuzimanja: 24* citiraj
APA
Hazler Pilepić, K., Friščić, M., Duran, A., Maslo, S., Garić, R., Čuljak, S., Šutalo, K. (2016). Contribution to Globularia phylogeny based on nuclear ribosomal spacer and two chloroplast DNA regions. Periodicum biologorum, 118(4). doi:10.18054/pb.v118i4.3856

Sažetak

Background and Purpose: Molecular approach has a major impact on phylogenetic studies of plants, considering that it gives useful information about evolutionary events and relations on all taxonomic levels. The sequence data of the nuclear ITS and of two chloroplast regions, trnL-trnF spacer and rbcL gene, obtained from thirteen Globularia L. taxa, including five Anatolian endemics, representing six sections altogether, were analyzed in order to determine the relations between the European and the Anatolian species and get a better insight into the phylogeny of several closely related Globularia taxa.

 

Materials and Methods: Total cellular DNA was extracted from fresh or frozen leaf tissue of thirteen Globularia samples. The ITS regions of nuclear DNA and two chloroplast DNA regions were amplified and sequenced. Obtained nuclear and combined plastid data matrices were subjected to Maximum Parsimony analyses.

 

Results and Conclusions: Molecular data that were obtained in this study indicate the existence of separate centers of diversification for the European and the Anatolian Globularia. The results provide support for relationships among the studied Anatolian endemic species and indications for a redefinition of affinities of some of the European species. The results presented herein are discussed along with available morphological, karyological, phytogeographical and molecular data.

 

Keywords: Globularia, ITS, trnL-trnF, rbcL, Maximum Parsimony, Quaternary

Hrčak ID: 177549

URI
http://hrcak.srce.hr/177549

Posjeta: 44 *