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Acta Pharmaceutica, Vol.67 No.3 Rujan 2017.

Izvorni znanstveni članak
DOI: 10.1515/acph-2017-0029

Development of new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples

JUMAH MASOUD MOHAMMAD SALMANI ; Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School Southeast University, Nanjing, People’s Republic of China
XUE WU ; Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School Southeast University, Nanjing, People’s Republic of China
JOE ANTONY JACOB ; Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School Southeast University, Nanjing, People’s Republic of China
RONG FU ; State Key Laboratory of Natural Medicines, Jiangsu Key Laboratory of Carcinogenesis and Intervention, China Pharmaceutical University, Nanjing, People's Republic of China
BAOAN CHEN ; Department of Hematology and Oncology (Key Department of Jiangsu Medicine), Zhongda Hospital, Medical School Southeast University, Nanjing, People’s Republic of China

Puni tekst: engleski, pdf (527 KB) str. 373-384 preuzimanja: 0* citiraj
APA
SALMANI, J.M.M., WU, X., JACOB, J.A., FU, R., CHEN, B. (2017). Development of new HPLC method for wogonin in rat plasma: Compatibility of standard and test samples. Acta Pharmaceutica, 67(3). doi:10.1515/acph-2017-0029

Sažetak
In the current paper, an HPLC/UV method was developed and validated for determination of wogonin in plasma. Considerable attention was paid to the preparation of standard samples and factors affecting drug distribution. A preparation procedure was devised to simulate the conditions the drug is expected to experience in vivo while pointing to the shortcomings of previously published methods. The method was validated according to the FDA regulations and showed to be highly efficient and capable of extracting the drug and IS from the plasma accurately and precisely within the specified range of 50–500 ng mL–1. Further, the standard sample preparation of this method can be used as a guideline for other methods, particularly when highly hydrophobic drugs with considerable protein binding are involved and could be valuable in the field of bioanalysis to improve the reliability of methods.

Ključne riječi
wogonin; HPLC/UV; standard sample preparation; sample compatibility

Hrčak ID: 183017

URI
http://hrcak.srce.hr/183017

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