Izvorni znanstveni članak
Partitioning of Phenylalanine Ammonia-lyase from Rhodotorula glutinis in Aqueous Two-phase Systems of PEG/salts
WY. Wang
; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
HY. Yue
; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
QP. Yuan
; State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
WC. Wang
; College of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, China
Sažetak
In this work, the partitioning of phenylalanine ammonia-lyase was investigated in different systems of PEG1000, PEG2000, PEG3350, PEG6000 and PEG8000 with (NH4)2SO4, Na2SO4, Na2CO3 and potassium phosphate. The results showed that the partition of phenylalanine ammonia-lyase in PEG/salt aqueous two-phase systems was mainly influenced by hydrophobicity, excluded volume effect and salting-out effect; compared with other PEG/salt systems, PEG1000/Na2SO4 was the most effective for phenylalanine ammonia-lyase partitioning. The result further supports that PEG/salt
aqueous two-phase system provides a new and applicable route for the purification of phenylalanine ammonia-lyase, as shown in our published paper.13
Ključne riječi
Aqueous two-phase system; phenylalanine ammonia-lyase; partitioning; PEG; salt
Hrčak ID:
62716
URI
Datum izdavanja:
27.12.2010.
Posjeta: 809 *