Acta Botanica Croatica, Vol. 47 No. 1, 1988.
Izvorni znanstveni članak
Cytochemical Localization of the Activity of Photosystem II in Bean Etiochloroplasts
Mercedes Wrischer
; Hrvatska
Sažetak
Photoreduction of thiocarbamyl nitroblue tetrazolium (TCNBT) was used to detect ultrastructurally the activity of photosystem II in the thylakoids of etiochloroplasts in greening bean leaves. To increase the retention of TCNBT forma- zan and consequently the contrast in the thylakoids, the samples were incubated in TCNBT and then treated by the 0s04-thiocarbohydrazide-0s04 method.
Dark deposits of TCNBT formazan appeared at first in the granal initials of the etiochloroplasts illuminated for 3 hours. The deposits were at the beginning scarce, but became more abundant after 6 and more hours of greening of the leaves. In young chloroplasts maintained in light for 24 or 48 hours the formazan deposits were located mostly in the partitions of the grana, although some scattered granules were attached also to the stroma thylakoids. There were no formazan deposits in the plastids of samples incubated only in darkness or in light with the addition of dichlorophenyl- dimethylurea.
Ključne riječi
Hrčak ID:
159433
URI
Datum izdavanja:
31.12.1988.
Posjeta: 745 *