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Original scientific paper
https://doi.org/10.5562/cca2902

Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies

Tin Weitner ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Suzana Inić ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Jasna Jablan ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Mario Gabričević ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia
Ana-Marija Domijan   ORCID icon orcid.org/0000-0001-5645-9732 ; Faculty of Pharmacy and Biochemistry, University of Zagreb, Ante Kovačića 1, 10000 Zagreb, Croatia

Fulltext: english, pdf (1 MB) pages 133-139 downloads: 2.077* cite
APA 6th Edition
Weitner, T., Inić, S., Jablan, J., Gabričević, M. & Domijan, A. (2016). Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies. Croatica Chemica Acta, 89 (1), 133-139. https://doi.org/10.5562/cca2902
MLA 8th Edition
Weitner, Tin, et al. "Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies." Croatica Chemica Acta, vol. 89, no. 1, 2016, pp. 133-139. https://doi.org/10.5562/cca2902. Accessed 11 Aug. 2020.
Chicago 17th Edition
Weitner, Tin, Suzana Inić, Jasna Jablan, Mario Gabričević and Ana-Marija Domijan. "Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies." Croatica Chemica Acta 89, no. 1 (2016): 133-139. https://doi.org/10.5562/cca2902
Harvard
Weitner, T., et al. (2016). 'Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies', Croatica Chemica Acta, 89(1), pp. 133-139. https://doi.org/10.5562/cca2902
Vancouver
Weitner T, Inić S, Jablan J, Gabričević M, Domijan A. Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies. Croatica Chemica Acta [Internet]. 2016 [cited 2020 August 11];89(1):133-139. https://doi.org/10.5562/cca2902
IEEE
T. Weitner, S. Inić, J. Jablan, M. Gabričević and A. Domijan, "Spectrophotometric Determination of Malondialdehyde in Urine Suitable for Epidemiological Studies", Croatica Chemica Acta, vol.89, no. 1, pp. 133-139, 2016. [Online]. https://doi.org/10.5562/cca2902

Abstracts
A reliable method for spectrophotometric determination of urinary malondialdehyde (MDA), according to the thiobarbituric acid (TBA) assay, is described. To account for matrix interference and differences in individual urine composition, standard addition procedure was applied. The method is adequately selective (LoQ = 0.09 μM in the presence of 0.1 M creatinine and 0.5 M urea) and reliable (within-day and between-day variability of less than 5 %). The mean level of urinary MDA was 1.52 ± 0.73 µM that is in good agreement with spectrofluorometric determination (1.20 ± 0.56 μM; p = 0.085) as well as with previous studies that used HPLC. Furthermore, it is demonstrated that MDA is stabile in urine at room temperature for 24 h and when stored at –20 °C for 6 months. The described method enables simple, rapid and cost-effective determination of urinary MDA as a relevant and non-invasive marker of “whole-body” oxidative stress.

Creative Commons License This work is licensed under a Creative Commons Attribution 4.0 International License.

Keywords
malondialdehyde; thiobarbituric acid assay; urine; interferences; stability

Hrčak ID: 160091

URI
https://hrcak.srce.hr/160091

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