Izvorni znanstveni članak
https://doi.org/10.17113/ftb.55.01.17.4693
Three New Lactobacillus plantarum Strains in the Probiotic Toolbox against Gut Pathogen Salmonella enterica Serotype Typhimurium
Mia Potočnjak
; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Petra Pušić
; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Jadranka Frece
; University of Zagreb, Faculty of Food Technology and Biotechnology, Laboratory for General Microbiology and Food Microbiology, Pierottijeva 6, HR-10000 Zagreb, Croatia
Maja Abram
; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Tamara Janković
; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Ivana Gobin
orcid.org/0000-0002-8956-4675
; University of Rijeka, Faculty of Medicine, Department of Microbiology and Parasitology, Braće Branchett a 20, HR-51000 Rijeka, Croatia
Sažetak
The benefits of probiotic bacteria have been widely explored. However, fermented foods and digestive system of humans and animals are an inexhaustible source of new potentially probiotic microorganisms. In this study we present three new Lactobacillus plantarum strains isolated from different dairy products: cow′s cheese, sheep′s cheese and whey. In order to determine the antibacterial activity of yet unexplored L. plantarum strains against Salmonella enterica serotype Typhimurium, in vitro competition and co-culture tests were done. Furthermore, adhesion of these strains to Caco-2 cells and their influence on the adhesion of Salmonella were tested. Results showed the potential probiotic activity of isolated strains. L. plantarum strains survived in the presence of 1 % bile salts, they possessed acidification ability, antibacterial activity and significantly attenuated the growth of S. Typhimurium in brain heart infusion broth. All tested L. plantarum strains were able to adhere to Caco-2 cells and significantly impair the adhesion of S. Typhimurium. All three L. plantarum strains exhibited signifi cant probiotic potential and anti-Salmonella activity; therefore, further testing on in vivo models should follow.
Ključne riječi
probiotic bacteria; antagonistic activity; Salmonella; mechanism of action
Hrčak ID:
178268
URI
Datum izdavanja:
23.3.2017.
Posjeta: 1.648 *