Izvorni znanstveni članak
https://doi.org/10.13044/j.sdewes.d6.0222
Bacterial Community Structure in Anaerobic Digesters of a Full Scale Municipal Wastewater Treatment Plant ‒ Case Study of Dubai, United Arab Emirates
Munawwar A. Khan
orcid.org/0000-0001-5683-300X
; Department of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, P.O. Box: 19282, Dubai, United Arab Emirates
Noorun N. Ashar
; School of Life Sciences, Manipal University, Dubai International Academic City, P.O. Box: 345050, Dubai, United Arab Emirates
Arpitha G. Ganesh
; Department of Life and Environmental Sciences, College of Natural and Health Sciences, Zayed University, P.O. Box: 19282, Dubai, United Arab Emirates
Naushad Rais
; School of Life Sciences, Manipal University, Dubai International Academic City, P.O. Box: 345050, Dubai, United Arab Emirates
Sultan M. Faheem
; School of Life Sciences, Manipal University, Dubai International Academic City, P.O. Box: 345050, Dubai, United Arab Emirates
Shams T. Khan
; Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh, Uttar Pradesh 2002002, India
Sažetak
A highly complex microbial community involved in anaerobic sludge digesters plays vital roles in sludge treatment. The data on microbial ecology is important to accomplish efficient operation of the anaerobic digesters. This study is aimed at monitoring the bacterial community of three full-scale anaerobic digesters of a full-scale municipal wastewater treatment Plant in Dubai, United Arab Emirates. Fluorescent in-situ
hybridization technique was applied to identify the bacterial groups and quantitative polymerase chain reaction to compare the richness of bacterial and archaeal domain. Results of the fluorescent in-situ hybridization technique analysis showed that the phylum Proteobacteria was most abundant followed by cytophage-Flavobacterium
group of Bacteroides, Firmicutes and Actinobacteria. Among proteobacterial subclass Delta- and Alpha- were dominating than Gamma- and Beta-proteobacteria. The genus Desulfobacter and Desulfobacterium were the dominant groups hybridizing 70-76% of
total 4’, 6’‒ diamidino – 2 phenylindole stained cells. The quantitative polymerase chain reaction results showed that Bacterial domain was dominating in all three digesters compared to the archaeal domain.
Ključne riječi
Anaerobic digester; Bacterial community; Fluorescence in situ hybridization; Oligonucleotide probes; Quantitative polymerase chain reaction.
Hrčak ID:
223987
URI
Datum izdavanja:
30.9.2019.
Posjeta: 1.073 *