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https://doi.org/10.37427/botcro-2020-026

De novo transcriptome analysis of high growth rate Pyropia yezoensis (Bangiales, Rhodophyta) mutant with high utilization of nitrogen

Seo-jeong Park ; Chonnam National University, Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy and Biomaterials, Gwangju 61186, South Korea
Jong-il Choi ; Chonnam National University, Department of Biotechnology and Bioengineering, Interdisciplinary Program for Bioenergy and Biomaterials, Gwangju 61186, South Korea


Puni tekst: engleski pdf 2.466 Kb

str. 201-211

preuzimanja: 298

citiraj


Sažetak

Pyropia yezoensis (Ueda) M.S.Hwang et H.G.Choi (Bangiales, Rhodophyta) has potentially high economic value. P. yezoensis has been used as food in East Asian countries for a long time, and, in addition to that its consumption is increasing worldwide owing to the growing interest in healthy seaweed food. A mutant (Py2K) with a high growth rate was developed using gamma rays to increase the production of P. yezoensis. De novo transcriptome analysis was performed to determine the mechanism underlying the high growth rate of this mutant. The transcriptomes from wild-type (PyWT) and mutant (Py2K) strains were assembled, and 167,165 genes were analyzed. A total of 15,979 genes were differentially expressed. Transcriptome analysis of nitrogen pathway revealed the increase in nitrogen availability through the upregulation of nitrate transporter gene (nrt) expression. Activation of nitrogen assimilation and re-assimilation and upregulation in alternative oxidase (aox) gene contributed to the increase in cellular nitrogen availability, thereby affecting the synthesis of phycobiliprotein. As a consequence, the efficiency of photosynthesis and the subsequent growth rate increased, which contributed to the color differences in thalli between PyWT and Py2K.

Ključne riječi

differentially expressed genes, high growth rate mutant, Pyropia yezoensis, RNA-sequencing, nitrogen availability, transcriptome analysis

Hrčak ID:

240846

URI

https://hrcak.srce.hr/240846

Posjeta: 635 *