hrcak mascot   Srce   HID

Review article
https://doi.org/10.18054/pb.v121-122i1-2.10867

Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents

Tena Čadež   ORCID icon orcid.org/0000-0002-1301-6816 ; Institute for Medical Research and Occupational Health, Ksaverska cesta 2, Zagreb, Croatia
Zrinka Kovarik   ORCID icon orcid.org/0000-0001-9863-886X ; Institute for Medical Research and Occupational Health, Zagreb

Fulltext: english, pdf (663 KB) pages 55-63 downloads: 117* cite
APA 6th Edition
Čadež, T. & Kovarik, Z. (2020). Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents. Periodicum biologorum, 121-122 (1-2), 55-63. https://doi.org/10.18054/pb.v121-122i1-2.10867
MLA 8th Edition
Čadež, Tena and Zrinka Kovarik. "Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents." Periodicum biologorum, vol. 121-122, no. 1-2, 2020, pp. 55-63. https://doi.org/10.18054/pb.v121-122i1-2.10867. Accessed 26 Oct. 2021.
Chicago 17th Edition
Čadež, Tena and Zrinka Kovarik. "Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents." Periodicum biologorum 121-122, no. 1-2 (2020): 55-63. https://doi.org/10.18054/pb.v121-122i1-2.10867
Harvard
Čadež, T., and Kovarik, Z. (2020). 'Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents', Periodicum biologorum, 121-122(1-2), pp. 55-63. https://doi.org/10.18054/pb.v121-122i1-2.10867
Vancouver
Čadež T, Kovarik Z. Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents. Periodicum biologorum [Internet]. 2020 [cited 2021 October 26];121-122(1-2):55-63. https://doi.org/10.18054/pb.v121-122i1-2.10867
IEEE
T. Čadež and Z. Kovarik, "Advancements in recombinant technology for production of butyrylcholinesterase, a bioscavenger of nerve agents", Periodicum biologorum, vol.121-122, no. 1-2, pp. 55-63, 2020. [Online]. https://doi.org/10.18054/pb.v121-122i1-2.10867

Abstracts

Butyrylcholinesterase (BChE) is a serine hydrolase present in plasma and other mammalian tissues. As a target of organophosphorous pesticides and warfare nerve agents, BChE acts as their stoichiometric bioscavenger. However, so far it has been a significant challenge to produce BChE at large scales and low cost. For decades, numerous research efforts have been directed first at isolation from human volunteers and later at production of BChE in eukaryotic and prokaryotic expression systems. In this review we focused on recent studies on recombinant BChE discussing reasons why the efficient, economically sensible expression system for recombinant butyrylcholinesterase is hard to develop. We also bring the most recent advancements in the use of expression of human BChE in vivo as an effective prophylactic against organophosphate poisoning.

Hrčak ID: 254648

URI
https://hrcak.srce.hr/254648

Visits: 179 *