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Original scientific paper

Propolis and its flavonoid compounds cause cytotoxicity on human urinary bladder transitional cell carcinoma in primary culture

NADA ORŠOLIĆ ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, Zagreb, Croatia
DAMIR ŠTAJCAR ; General Hospital, Urology Department, Meštrovićeva b.b., 42000 Varaždin, Croatia
IVAN BAŠIĆ ; Department of Animal Physiology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, Zagreb, Croatia

Fulltext: english, pdf (764 KB) pages 113-121 downloads: 1.756* cite
APA 6th Edition
ORŠOLIĆ, N., ŠTAJCAR, D. & BAŠIĆ, I. (2009). Propolis and its flavonoid compounds cause cytotoxicity on human urinary bladder transitional cell carcinoma in primary culture. Periodicum biologorum, 111 (1), 113-121. Retrieved from https://hrcak.srce.hr/36004
MLA 8th Edition
ORŠOLIĆ, NADA, et al. "Propolis and its flavonoid compounds cause cytotoxicity on human urinary bladder transitional cell carcinoma in primary culture." Periodicum biologorum, vol. 111, no. 1, 2009, pp. 113-121. https://hrcak.srce.hr/36004. Accessed 14 Nov. 2019.
Chicago 17th Edition
ORŠOLIĆ, NADA, DAMIR ŠTAJCAR and IVAN BAŠIĆ. "Propolis and its flavonoid compounds cause cytotoxicity on human urinary bladder transitional cell carcinoma in primary culture." Periodicum biologorum 111, no. 1 (2009): 113-121. https://hrcak.srce.hr/36004
Harvard
ORŠOLIĆ, N., ŠTAJCAR, D., and BAŠIĆ, I. (2009). 'Propolis and its flavonoid compounds cause cytotoxicity on human urinary bladder transitional cell carcinoma in primary culture', Periodicum biologorum, 111(1), pp. 113-121. Available at: https://hrcak.srce.hr/36004 (Accessed 14 November 2019)
Vancouver
ORŠOLIĆ N, ŠTAJCAR D, BAŠIĆ I. Propolis and its flavonoid compounds cause cytotoxicity on human urinary bladder transitional cell carcinoma in primary culture. Periodicum biologorum [Internet]. 2009 [cited 2019 November 14];111(1):113-121. Available from: https://hrcak.srce.hr/36004
IEEE
N. ORŠOLIĆ, D. ŠTAJCAR and I. BAŠIĆ, "Propolis and its flavonoid compounds cause cytotoxicity on human urinary bladder transitional cell carcinoma in primary culture", Periodicum biologorum, vol.111, no. 1, pp. 113-121, 2009. [Online]. Available: https://hrcak.srce.hr/36004. [Accessed: 14 November 2019]

Abstracts
Background and purpose: The flavonoids present in propolis are considered to be a rich source of chemopreventive agents since they have various therapeutic biological activities. This study was carried out to find whether propolis and its polyphenolic/flavonoid compounds may induce cytotoxicity in primary culture of human urinary bladder transitional cell carcinoma (TCC) cells as compared to normal urinary bladder epithelial cells.

Material and Methods: Pieces of TCC or normal bladder epithelial tissue were collected by transurethral surgery from patients in different stages (grade G1, G2, G3) of TCC. Single cell suspension from pieces of either TCC or normal bladder epithelial tissue was prepared according to standard laboratory procedure. Incubation for cytotoxicity testing was carried out in RPMI-1640 medium with 20% FCS at 37°C and with 5% CO2 with or without different concentration (50, 150, 300 μg/ml) of test components.

Results:The cytotoxicity of two preparations of propolis (water and
ethanolic extract of propolis; WSDP or EEP) and its polyphenolic compounds (caffeic acid, naringin, chrysin, and quercetin) was determined using trypan blue exclusion assay. The findings suggest that EEP is the most effective in inhibition of urinary bladder TCC cell proliferation as compared to WSDP or single flavonoids derived from propolis. All test components showed no cytotoxic effect on normal epithelial cells.

Conclusions: The result of this study may have considerable impact on the potential use of EEP as an adjuvant to surgery to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. As immunomodulation by BCG has been used to improve the results of surgery it is likely that propolis preparation (EEP) as an imunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation or its polyphenolic components have expressed no side effect after treatment. However, the exact cancer chemoprevention mechanisms of propolis have to be elucidated.

Keywords
Propolis; Flavonoids, Human urinary bladder cancer, Cytotoxicity, Primary culture

Hrčak ID: 36004

URI
https://hrcak.srce.hr/36004

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