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Antioxidant Activity of Phytic Acid in Lipid Model System

Marijana Sakač ; Institut za prehrambene tehnologije, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Jasna Čanadanović-Brunet ; Katedra za primenjene i inženjerske hemije, Tehnološki fakultet, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Aleksandra Mišan ; Institut za prehrambene tehnologije, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Vesna Tumbas ; Katedra za primenjene i inženjerske hemije, Tehnološki fakultet, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija
Đorđe Medić ; Institut za prehrambene tehnologije, Univerzitet u Novom Sadu, Bulevar cara Lazara 1, RS-21000 Novi Sad, Srbija


Puni tekst: engleski pdf 442 Kb

str. 524-529

preuzimanja: 1.287

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Sažetak

Free radicals formed during thermal (60 °C, 24 h) and catalytic (Fe2+ ions, room temperature (23±1) °C, 3 h) oxidative degradation of hydroperoxyde-enriched soybean oil (HESO) were stabilized in the presence of spin trap N-tert-butyl-α-phenylnitrone (PBN) and detected by electron spin resonance (ESR) spectrometry. In both thermal and catalytic oxidation of HESO, the same hyperfine coupling parameters (aN=14.75 G and aH β=2.80 G) confirmed the generation of PBN-OOL/-OL spin adducts. The antiradical activity (AA) of phytic acid, in the 0.076–0.30 mM concentration range, was tested by measuring its ability to inhibit the generation of PBN-OOL/-OL spin adducts during thermal and catalytic oxidation of HESO. Phytic acid did not inhibit the thermal oxidation of HESO and showed no effect in the β-carotene bleaching test (AOA). Contrary to this, phytic acid exhibited antioxidant effect on the catalytic oxidation of HESO by chelating Fe2+ ions. The mechanism of antioxidant activity was confirmed by the results of chelating activity on Fe2+ in Fe2+-ferrozine test where a dose-dependent chelating activity of phytic acid was obtained.

Ključne riječi

phytic acid; lipid oxyradicals; antioxidant activity; ESR; chelating activity

Hrčak ID:

61724

URI

https://hrcak.srce.hr/61724

Datum izdavanja:

15.12.2010.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.985 *