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https://doi.org/10.2478/10004-1254-62-2011-2106

Elimination of Chloramphenicol in Rainbow Trout Receiving Medicated Feed

Nina Bilandžić orcid id orcid.org/0000-0002-0009-5367 ; Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia
Ivana Varenina ; Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia
Sanin Tanković ; Veterinary Offi ce of Bosnia and Herzegovina, Sarajevo, Bosnia and Herzegovina
Božica Solomun Kolanović ; Department of Veterinary Public Health, Laboratory for Residue Control, Croatian Veterinary Institute, Zagreb, Croatia


Puni tekst: engleski pdf 85 Kb

str. 215-219

preuzimanja: 1.024

citiraj


Sažetak

Chloramphenicol muscle residue levels in rainbow trout were determined after oral administration of 84 μg kg−1d−1 of chloramphenicol for four days. Samples were taken one day before treatment and for 43 days after the treatment was over. Chloramphenicol was analysed using an in-house enzyme linked
immunoassay (ELISA) validated against the criteria of the Commission Decision 2002/657/EC. Validation parameters confi rmed that the method was appropriate for the detection of chloramphenicol at levels below the minimum required performance limit (MRPL) of 0.3 μg kg−1. The highest chloramphenicol levels were
observed on the fi rst day after the treatment had ended (144.3 μg kg−1). Elimination was signifi cant over the fi rst seven days; signifi cant differences were detected between days 1 and 3 (p<0.001), 3 and 5 (p<0.001), and 5 and 7 (p<0.05). Chloramphenicol levels dropped below MRPL to 0.17 μg kg−1 on day 9 after the end
of treatment. From day 11 to 43, chloramphenicol residues were detectable in a range from 0.091 μg kg−1 (highest) to 0.011 μg kg−1 (lowest). Our results indicate that trout muscle tissue could be compliant with health requirements for consumption 10 days after withdrawal from chloramphenicol treatment.

Ključne riječi

antibiotic; ELISA; freshwater fish; residue

Hrčak ID:

71758

URI

https://hrcak.srce.hr/71758

Datum izdavanja:

23.9.2011.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.813 *