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Tetrahydrophthalazine Derivative »Sodium Nucleinate« Exert its Anti-Inflammatory Effects through Inhibition of Oxidative Burst in Human Monocytes
Tomislav Jukić
Alojz Ihan
Dubravko Jukić
Sažetak
We described the use of a new chemical substance Sodium nucleinate (SN) as an immunomodulatory substance exhibiting
antiinflammatory properties. Sodium nucleinate (SN) registrated in Russian Federation as Tamerit®, is 2-amino-
-1,2,3,4-tetrahydrophthalazine-1,4-dione sodium salt dihydrate, derivative of well known chemical substance luminol.
To comprehend the mechanisms of SN immunomodulatory activity, we examined the SN modulation of the oxidative
burst responses of whole blood human monocytes and polimorphonuclear cells (PMC) stimulated with phorbol 12-myristate
13-acetate (PMA) or E. coli suspension in vitro. SN did not inhibit the proportion of neutrophils and monocytes
phagocytosing E. coli. Oxidative burst responses of monocytes stimulated with PMA were strongly inhibited at SN concentration
ranging from 10–500 mg/ml, less efficient inhibitor was SN in E. coli stimulated monocytes (inhibition range
was from 50–500 mg/ml SN). SN inhibited PMC oxidative burst only in range 100–500 mg/ml SN. In conclusion, we
found SN as an efficient inhibitor of oxidative burst in monocytes. Since ROS generation in monocytes/macrophages has
been found to be important for LPS-driven production of several proinflammatory cytokines, SN may exsert its antiinflammatory
effects through monocyte/macrophage oxidative burst inhibition.
Ključne riječi
oxidative burst; granulocytes; monocytes; Sodium nucleinate
Hrčak ID:
84777
URI
Datum izdavanja:
10.7.2012.
Posjeta: 808 *