Veterinarski arhiv, Vol. 71 No. 6, 2001.
Izvorni znanstveni članak
Differentiation of infectious bursal disease virus strains at a genomic level.
Zdenko Biđin
; Department of Poultry Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Ivana Lojkić
; Department of Poultry Pathology, Faculty of Veterinary Medicine, University of Zagreb, Zagreb, Croatia
Magdalena Grce
; Division of Molecular Medicine, Institute Ruđer Bošković, Zagreb, Croatia
Stanislav Čajavec
; Veterina d.o.o., Kalinovica, Croatia
Biserka Pokrić
; Division of Molecular Medicine, Institute Ruđer Bošković, Zagreb, Croatia
Sažetak
Reverse transcription-polymerase chain reaction (RT-PCR) followed by restriction fragment-length polymorphism (RFLP) analysis was tested in order to determine its accuracy and sensitivity for differentiation of infectious bursal disease virus (IBDV) strains. Two commercial vaccine strains and two Croatian field isolates were analysed. RT-PCR assay was used for amplification of a variable part of VP2 gene (422 bp) encoding for major neutralizing epitopes. Amplified VP2 fragments were further characterized by RFLP analysis using the SacI and TaqI restriction endonucleases. Both field isolates showed RFLP pattern similar to highly virulent European and Asian strains. Both vaccine strains had RFLP profiles different from that of the field isolates. Vaccinal strain 2512 had RFLP pattern similar to mild vaccinal strain pbg-98, and strain VMG-91 was similar to other classical vaccinal strains of intermediate pathogenicity.
Ključne riječi
infectious bursal disease; RT-PCR; RFLP; vaccinal strains; field isolates
Hrčak ID:
90126
URI
Datum izdavanja:
19.12.2001.
Posjeta: 1.664 *