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Influence of Application Time of Antioxidant on the Deproteinized Dentin: a SEM/EDS Study

Ana Isabella Arruda MEIRA RIBEIRO ; School of Dentistry, State University of Paraíba, Campina Grande, PB, Brazil
Darlene Cristina Ramos Eloy DANTAS ; School of Dentistry, State University of Paraíba, Campina Grande, PB, Brazil
José Wrban Garcia da SILVA ; School of Dentistry, State University of Paraíba, Campina Grande, PB, Brazil
Gymenna Maria Tenório GUENES ; School of Dentistry, Federal University of Campina Grande, Patos, PB, Brazil
Rodivan BRAZ ; Faculty of Dentistry, University of Pernambuco, Camaragibe, PE, Brazil
Alessandro Leite CAVALCANTI ; School of Dentistry, State University of Paraíba, Campina Grande, PB, Brazil

Puni tekst: engleski, pdf (348 KB) str. 93-99 preuzimanja: 193* citiraj
APA 6th Edition
MEIRA RIBEIRO, A.I.A., DANTAS, D.C.R.E., SILVA, J.W.G.d., GUENES, G.M.T., BRAZ, R. i CAVALCANTI, A.L. (2011). Influence of Application Time of Antioxidant on the Deproteinized Dentin: a SEM/EDS Study. Acta stomatologica Croatica, 45 (2), 93-99. Preuzeto s https://hrcak.srce.hr/69060
MLA 8th Edition
MEIRA RIBEIRO, Ana Isabella Arruda, et al. "Influence of Application Time of Antioxidant on the Deproteinized Dentin: a SEM/EDS Study." Acta stomatologica Croatica, vol. 45, br. 2, 2011, str. 93-99. https://hrcak.srce.hr/69060. Citirano 28.09.2020.
Chicago 17th Edition
MEIRA RIBEIRO, Ana Isabella Arruda, Darlene Cristina Ramos Eloy DANTAS, José Wrban Garcia da SILVA, Gymenna Maria Tenório GUENES, Rodivan BRAZ i Alessandro Leite CAVALCANTI. "Influence of Application Time of Antioxidant on the Deproteinized Dentin: a SEM/EDS Study." Acta stomatologica Croatica 45, br. 2 (2011): 93-99. https://hrcak.srce.hr/69060
Harvard
MEIRA RIBEIRO, A.I.A., et al. (2011). 'Influence of Application Time of Antioxidant on the Deproteinized Dentin: a SEM/EDS Study', Acta stomatologica Croatica, 45(2), str. 93-99. Preuzeto s: https://hrcak.srce.hr/69060 (Datum pristupa: 28.09.2020.)
Vancouver
MEIRA RIBEIRO AIA, DANTAS DCRE, SILVA JWGd, GUENES GMT, BRAZ R, CAVALCANTI AL. Influence of Application Time of Antioxidant on the Deproteinized Dentin: a SEM/EDS Study. Acta stomatologica Croatica [Internet]. 2011 [pristupljeno 28.09.2020.];45(2):93-99. Dostupno na: https://hrcak.srce.hr/69060
IEEE
A.I.A. MEIRA RIBEIRO, D.C.R.E. DANTAS, J.W.G.d. SILVA, G.M.T. GUENES, R. BRAZ i A.L. CAVALCANTI, "Influence of Application Time of Antioxidant on the Deproteinized Dentin: a SEM/EDS Study", Acta stomatologica Croatica, vol.45, br. 2, str. 93-99, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/69060. [Citirano: 28.09.2020.]
Puni tekst: hrvatski, pdf (348 KB) str. 93-99 preuzimanja: 516* citiraj
APA 6th Edition
MEIRA RIBEIRO, A.I.A., DANTAS, D.C.R.E., SILVA, J.W.G.d., GUENES, G.M.T., BRAZ, R. i CAVALCANTI, A.L. (2011). Utjecaj duljine primjene antioksidanta na deproteinizirani dentin: istraživanje SEM/EDS-om. Acta stomatologica Croatica, 45 (2), 93-99. Preuzeto s https://hrcak.srce.hr/69060
MLA 8th Edition
MEIRA RIBEIRO, Ana Isabella Arruda, et al. "Utjecaj duljine primjene antioksidanta na deproteinizirani dentin: istraživanje SEM/EDS-om." Acta stomatologica Croatica, vol. 45, br. 2, 2011, str. 93-99. https://hrcak.srce.hr/69060. Citirano 28.09.2020.
Chicago 17th Edition
MEIRA RIBEIRO, Ana Isabella Arruda, Darlene Cristina Ramos Eloy DANTAS, José Wrban Garcia da SILVA, Gymenna Maria Tenório GUENES, Rodivan BRAZ i Alessandro Leite CAVALCANTI. "Utjecaj duljine primjene antioksidanta na deproteinizirani dentin: istraživanje SEM/EDS-om." Acta stomatologica Croatica 45, br. 2 (2011): 93-99. https://hrcak.srce.hr/69060
Harvard
MEIRA RIBEIRO, A.I.A., et al. (2011). 'Utjecaj duljine primjene antioksidanta na deproteinizirani dentin: istraživanje SEM/EDS-om', Acta stomatologica Croatica, 45(2), str. 93-99. Preuzeto s: https://hrcak.srce.hr/69060 (Datum pristupa: 28.09.2020.)
Vancouver
MEIRA RIBEIRO AIA, DANTAS DCRE, SILVA JWGd, GUENES GMT, BRAZ R, CAVALCANTI AL. Utjecaj duljine primjene antioksidanta na deproteinizirani dentin: istraživanje SEM/EDS-om. Acta stomatologica Croatica [Internet]. 2011 [pristupljeno 28.09.2020.];45(2):93-99. Dostupno na: https://hrcak.srce.hr/69060
IEEE
A.I.A. MEIRA RIBEIRO, D.C.R.E. DANTAS, J.W.G.d. SILVA, G.M.T. GUENES, R. BRAZ i A.L. CAVALCANTI, "Utjecaj duljine primjene antioksidanta na deproteinizirani dentin: istraživanje SEM/EDS-om", Acta stomatologica Croatica, vol.45, br. 2, str. 93-99, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/69060. [Citirano: 28.09.2020.]

Sažetak
Objective: To evaluate in vitro the influence of application time of sodium ascorbate to 10% on the deproteinized by scanning electron microscopy/energy dispersive x-ray spectroscopy (SEM/EDS). Methods: Seven extracted human third molars were selected. For analysis of the composition of the dentin surface by SEM/EDS, 7 samples were analyzed simultaneously as follows: G1: Healthy dentin
substrate, G2: Demineralized dentin substrate with 37% phosphoric acid for 15 s (demineralization), strictly following the manufacturer’s recommendations, G3: Deproteinized dentin substrate after demineralization, 10% sodium hypochlorite aqueous solution for 60 s under constant agitation (deproteinization), G4, G5, G6 and G7: demineralized, deproteinized dentin substrate after application of 10% sodium ascorbate for 15 s, 30 s, 60 s, 10 min, respectively. The samples were examined by a scanning electron microscope (Quanta 2000 - Fei Company) equipped with an energy dispersive spectrometer with acceleration voltage 10 KV at ×2,000 magnification under vacuum. Results: The SEM/EDS analysis showed progressive deposition of sodium ascorbate crystals as the application time of the antioxidant agent increased, forming crusts which can cause occlusion of some dentinal tubules. There was no statistically significant difference between G2 and G3 (P>0.05). However, the application of 10% sodium ascorbate for 60 s resulted in a decrease in the oxygen (P=0.029), magnesium (P=0.019) and sodium (P=0.029) levels. Conclusion: After application of 10% sodium ascorbate, deposition of sodium ascorbate crystals occurred progressively forming crusts on dentin substrate, which causes occlusion of some dentinal tubules.

Ključne riječi
Dentin; Antioxidants; Ascorbic Acid; Microscopy, Electron, Scanning; Spectrometry, X-Ray Emission

Hrčak ID: 69060

URI
https://hrcak.srce.hr/69060

[hrvatski]

Posjeta: 1.114 *