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DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS

Ivica Strelec   ORCID icon orcid.org/0000-0003-3999-8499 ; J.J. Strossmayer University of Osijek, Faculty of Food Technology - Franje Kuhača 20, HR-31000 Osijek, Croatia
Elzabeta Has-Schön ; Department of Biology, Gajev trg 6, HR-31000 Osijek, Croatia
Ljubinka Vitale ; Ruđer Bošković Institute, Bijenička cesta 54, HR-10000 Zagreb

Puni tekst: engleski, pdf (218 KB) str. 23-27 preuzimanja: 483* citiraj
APA 6th Edition
Strelec, I., Has-Schön, E. i Vitale, Lj. (2011). DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS. Poljoprivreda, 17 (1), 23-27. Preuzeto s https://hrcak.srce.hr/69540
MLA 8th Edition
Strelec, Ivica, et al. "DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS." Poljoprivreda, vol. 17, br. 1, 2011, str. 23-27. https://hrcak.srce.hr/69540. Citirano 19.09.2019.
Chicago 17th Edition
Strelec, Ivica, Elzabeta Has-Schön i Ljubinka Vitale. "DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS." Poljoprivreda 17, br. 1 (2011): 23-27. https://hrcak.srce.hr/69540
Harvard
Strelec, I., Has-Schön, E., i Vitale, Lj. (2011). 'DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS', Poljoprivreda, 17(1), str. 23-27. Preuzeto s: https://hrcak.srce.hr/69540 (Datum pristupa: 19.09.2019.)
Vancouver
Strelec I, Has-Schön E, Vitale Lj. DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS. Poljoprivreda [Internet]. 2011 [pristupljeno 19.09.2019.];17(1):23-27. Dostupno na: https://hrcak.srce.hr/69540
IEEE
I. Strelec, E. Has-Schön i Lj. Vitale, "DIFFERENTIATION OF CROATIAN BARLEY VARIETIES BY GRADIENT GEL SDS-PAGE AND ISOELECTRIC FOCUSING OF DRY GRAINS AND GREEN MALT HORDEINS", Poljoprivreda, vol.17, br. 1, str. 23-27, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/69540. [Citirano: 19.09.2019.]

Sažetak
Applicability of polyacrylamide gel electrophoresis and isoelectric focusing of hordeins for discrimination of six two-rowed winter barley varieties (Angora, Sladoran, Rodnik, Rex, Martin and Barun) has been investigated. Hordeins extracted from dry grains and green malt of barley varieties were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis in gradient gel of 8-18% T, and by isoelectric focusing in pH gradient of 3.5-9.5 and 5.5-8.5, respectively. In all separation experiments better resolution of proteins was achieved with dry grain extracts, than with malt extracts. Angora, Sladoran and Martin variety could be distinguished from other varieties by differences in hordein patterns obtained by gradient gel SDS-PAGE (8-18% T), and Angora, Sladoran, Martin and Rodnik by isoelectric focusing in pH gradient 5.5-8.5.

Ključne riječi
barley variety discrimination; gradient gel SDS-PAGE; hordeins; isoelectric focusing

Hrčak ID: 69540

URI
https://hrcak.srce.hr/69540

[hrvatski]

Posjeta: 741 *