hrcak mascot   Srce   HID

Izvorni znanstveni članak
https://doi.org/10.5562/cca1823

Pre-transfer Editing of Serine Hydroxamate within the Active Site of Methanogenic-type Seryl-tRNA Synthetase

Ita Gruić-Sovulj ; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000 Zagreb, Croatia
Morana Dulić ; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000 Zagreb, Croatia
Ivana Weygand-Đurašević ; Department of Chemistry, Faculty of Science, University of Zagreb, Horvatovac 102a, 10 000 Zagreb, Croatia

Puni tekst: engleski, pdf (4 MB) str. 179-184 preuzimanja: 629* citiraj
APA 6th Edition
Gruić-Sovulj, I., Dulić, M. i Weygand-Đurašević, I. (2011). Pre-transfer Editing of Serine Hydroxamate within the Active Site of Methanogenic-type Seryl-tRNA Synthetase. Croatica Chemica Acta, 84 (2), 179-184. https://doi.org/10.5562/cca1823
MLA 8th Edition
Gruić-Sovulj, Ita, et al. "Pre-transfer Editing of Serine Hydroxamate within the Active Site of Methanogenic-type Seryl-tRNA Synthetase." Croatica Chemica Acta, vol. 84, br. 2, 2011, str. 179-184. https://doi.org/10.5562/cca1823. Citirano 25.02.2021.
Chicago 17th Edition
Gruić-Sovulj, Ita, Morana Dulić i Ivana Weygand-Đurašević. "Pre-transfer Editing of Serine Hydroxamate within the Active Site of Methanogenic-type Seryl-tRNA Synthetase." Croatica Chemica Acta 84, br. 2 (2011): 179-184. https://doi.org/10.5562/cca1823
Harvard
Gruić-Sovulj, I., Dulić, M., i Weygand-Đurašević, I. (2011). 'Pre-transfer Editing of Serine Hydroxamate within the Active Site of Methanogenic-type Seryl-tRNA Synthetase', Croatica Chemica Acta, 84(2), str. 179-184. https://doi.org/10.5562/cca1823
Vancouver
Gruić-Sovulj I, Dulić M, Weygand-Đurašević I. Pre-transfer Editing of Serine Hydroxamate within the Active Site of Methanogenic-type Seryl-tRNA Synthetase. Croatica Chemica Acta [Internet]. 2011 [pristupljeno 25.02.2021.];84(2):179-184. https://doi.org/10.5562/cca1823
IEEE
I. Gruić-Sovulj, M. Dulić i I. Weygand-Đurašević, "Pre-transfer Editing of Serine Hydroxamate within the Active Site of Methanogenic-type Seryl-tRNA Synthetase", Croatica Chemica Acta, vol.84, br. 2, str. 179-184, 2011. [Online]. https://doi.org/10.5562/cca1823

Sažetak
Aminoacyl-tRNA synthetases (aaRSs) maintain fidelity of protein synthesis by matching only
cognate amino acid-tRNA pairs. Aminoacylation occurs through activation of amino acid to yield aminoacyl-
adenylate followed by transfer of acyl-moiety to tRNA. Error-prone aaRSs achieve high level of
accuracy using inherent hydrolytic activities towards noncognate aminoacyl-adenylate or misacylated
tRNA (pre- and post-transfer editing).
Seryl-tRNA synthetases can be divided into two structurally different types: canonical and methanogenic-
type. Both types have been shown to efficiently activate serine analogue serine hydroxamate
(SerHX). Moreover, this analogue has been also eliminated by pre-transfer editing within the canonical
synthetic site of yeast SerRS. Here we show that methanogenic-type SerRS from Methanosarcina barkeri
clears misactivated SerHX similarly as the yeast enzyme: SerHX-adenylate is not expelled into solution,
but is enzymatically hydrolyzed in a tRNA-independent manner. Since the enzyme lacks domain specialized
in editing, this shows that methanogenic-type catalytic core is also capable to perform pre-transfer
editing. (doi: 10.5562/cca1823)

Ključne riječi
editing; proofreading; serine hydroxamate; seryl-tRNA synthetase

Hrčak ID: 71980

URI
https://hrcak.srce.hr/71980

Posjeta: 920 *