Izvorni znanstveni članak
Use of SNP Markers Within the Fat Mass and Obesity-associated (FTO) Gene to Verify Pedigrees and Determine Haplotypes in Paternal Half-sib Families of Slovenian Simmental Cattle
Daša Jevšinek Skok
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Tanja Kunej
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Andrej Renčelj
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Andrej Razpet
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Peter Dovč
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Silvester Žgur
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Nežika Petrič
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Martina Planinc
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Špela Malovrh
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Milena Kovač
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia
Simon Horvat
; University of Ljubljana, Biotechnical faculty, Department of Animal Science, Groblje 3, SI-1230 Domžale, Slovenia; National Institute of Chemistry, Hajdrihova 19, SI-1001 Ljubljana, Slovenia
Sažetak
The objective of this preliminary study was to identify SNP markers within the FTO gene for evaluation of pedigree data accuracy and determination of haplotypes in paternal half-sib families of Slovenian Simmental cattle. Out of 23 polymorphic SNPs identified ten most informative SNPs for genotyping 31 sires and 56 half-sib progeny were used. The ATLAS program was used for paternity testing. Haplotype analysis revealed three haplotype blocks. The effect of SNPs “ex2 T>C” and “int2 indel*>T” was significant on three correlated carcass traits: live weight at slaughter (P= 0.03), carcass weight (P= 0.038), and lean weight (P= 0.048). The FTO gene can thus be regarded as a candidate for the marker assisted selection programs in our and possibly other populations of cattle. Future studies in cattle might also reveal novel roles of the FTO gene in carcass traits on livestock species as well as fatness control in other mammals.
Ključne riječi
cattle; growth; FTO; SNP; haplotype
Hrčak ID:
72110
URI
Datum izdavanja:
4.10.2011.
Posjeta: 942 *