Izvorni znanstveni članak
Effect of Hyperbaric Oxygen Treatment on Myogenic Transcriptional Factors in Regenerating Rat Masseter Muscle
Snježana Bajek
Marina Nikolić
Tamara Šoić-Vranić
Juraj Arbanas
Goran Bajek
Sažetak
Hyperbaric oxygen (HBO) treatment was shown to be beneficial as an additional treatment for ischemic muscles in
crush injuries and ischemia-reperfusion injuries. The purpose of this study was to assess the influence of hyperbaric oxygen
treatment on transcriptional myogenic factors during muscle regeneration. Those factors (MyoD, myf5, myogenin,
mrf4) are essential for determination and differentiation of skeletal muscle tissue and together with several other factors
control gene expression during myogenesis. The process of regeneration in rat masseter muscle was provoked with injection
of local anesthetic bupivacaine hydrochloride. Following injection, the animals were treated once daily in hyperbaric
chamber from one to ten days and than sacrificed. Immunohistochemical and Western blot analysis of frozen masseter
muscle samples showed a transient upregulation of myoD and myogenin transcriptional factors in the muscles of
hyperbaric oxygen treated rats and of rats that have not been treated after the injury. HBO treatment had no effect on the
expression of MyoD and myogenin transcriptional factors in the regenerating rat masseter muscle.
Ključne riječi
hyperbaric oxygen treatment; rat muscle; regeneration; myogenic regulatory factors
Hrčak ID:
72238
URI
Datum izdavanja:
25.9.2011.
Posjeta: 866 *