Izvorni znanstveni članak
Determination of Enzyme Matrix Metalloproteinases-9 and Immune Status as Indicators of Development of the Environmental Diseases
Aleksandar Bulog
Vladimir Mićović
Petra Šuljić
Ines Mrakovčić-Šutić
Sažetak
The majority of environmental diseases are multifactorial airway illnesses, including genetic background and exposure
to different kind of airborne irritants and allergens. Altered lifestyle and changes in environmental exposures contribute
to the occurring of these diseases. The term of environmental illnesses includes the disease primarily caused by
pollution of air and water, chemical and physical agents, radiation, contaminated food and direct contact with the toxins
we are exposed to natural and/or working environment. The members of the matrix metalloproteinase (MMP) family are
involved in the pathogenesis of COPD. MMPs comprise a large family of structurally related zinc metalloendopeptidases
with different substrate specificities and possibilities to degrade protein constituents of the extracellular matrix. We investigated
immunological status and level of MMP-9 in workers occupationally exposed to volatile aromatic hydrocarbons
compared to urban residents and rural areas. The phenotypic profiles of peripheral blood lymphocytes were done by
flow cytometry. The method of enzyme immunoassay (ELISA) was used to determine enzyme expression of matrix
metalloproteinase-9 (MMP-9). The occupationally exposed group had a significantly elevated level of enzyme MMP-9 in
the urine, accompanied with augmentation of cells of innate immunity in peripheral blood, which could contribute to the
monitoring, early detection of environmental diseases and consequent earlier and more effective treatment.
Ključne riječi
environmental disease; enzyme metalloproteinase-9 (MMP-9); innate immunity; NKT cells
Hrčak ID:
72255
URI
Datum izdavanja:
25.9.2011.
Posjeta: 818 *