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Increased DAPK1 but decreased CCL2 plasma levels of nucleic acids in patients with stable angina

Darko Cerne ; Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Irma Stern ; Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia
Igor Kranjec ; Department of Cardiology, University Medical Centre Ljubljana, Ljubljana, Slovenia
Andreja Cerne ; Department of Cardiology, University Medical Centre Ljubljana, Ljubljana, Slovenia
Darko Zorman ; Department of Cardiology, University Medical Centre Ljubljana, Ljubljana, Slovenia
Simona Krzisnik-Zorman ; Department of Cardiology, University Medical Centre Ljubljana, Ljubljana, Slovenia
Janja Marc ; Department of Clinical Biochemistry, Faculty of Pharmacy, University of Ljubljana, Ljubljana, Slovenia


Puni tekst: engleski pdf 137 Kb

str. 291-296

preuzimanja: 713

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Sažetak

Introduction: We hypothesized that patients with stable angina have increased plasma levels of mRNA from genes responsible for atherosclerotic plaque development and destabilisation, i.e. from death-associated protein kinase (DAPK1) and monocyte chemotactic protein-1 (CCL2).
Materials and methods: Nucleic acids were isolated from plasma of patients with stabile angina and healthy subjects as controls. mRNAs were transcribed to cDNAs, quantified by real-time PCR and standardized to the amount of a reference gene. Reagents for PCR quantification are declared to be mRNA specific, but in our test conditions DNA was found to interfere in both assays.
Results: Patients had 5.1-times higher plasma level of DAPK1 nucleic acids (mRNA and DNA) than controls (P < 0.001) and the highest levels were associated with the presence of diabetes. However, plasma levels of CCL2 tended to be lower than in controls, and in statin-treated patients the decre-ment reached significance (-66.3%; P = 0.041).
Conclusion: The estimated levels are explicable in terms of current knowledge. Further studies with specific assays for mRNA PCR quantification are reasonable to access whether this approach offers non-invasive in vivo assessment and monitoring of gene expression profile in atherosclerotic vascular beds.

Ključne riječi

DAPK1; CCL2; cell-free nucleic acids; plasma; stable angina

Hrčak ID:

72950

URI

https://hrcak.srce.hr/72950

Datum izdavanja:

15.10.2011.

Posjeta: 1.289 *