Izvorni znanstveni članak

Immunosuppressive effect of combined citrinin and endosulfan toxicity in pregnant Wistar rats.

Nittin D. Singh ; Department of Veterinary Pathology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Anil K. Sharma ; Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, India
Prabhaker Dwivedi ; Division of Pathology, Indian Veterinary Research Institute, Izatnagar, Bareilly, India
Manoj Kumar ; High Security Animal Disease Laboratory, Bhopal, India
Rajendra D. Patil ; Department of Veterinary Pathology, College of Veterinary Science, Himachal Pradesh Krishi Vishwa Vidyalaya, Palampur, India

Sažetak

In the present investigation, citrinin (CIT) (10 mg/kg feed) and endosulfan (1mg/kg bw) were administered orally alone and in combination to pregnant Wistar rats from gestational day 6 to 20 to study their effect on the immune response of the pregnant Wistar rats. Cell mediated Immune response was assessed by delayed type hypersensitivity against ovalbumin, and lymphocyte transformation test (LTT) using MTT (3-(4,5-dimethyl thiazol-2-yl) 2, 5-diphenyl tetrazolium bromide). The humoral immune response was judged by Hemagglutination against SRBCs and indirect ELISA. Cell mediated immune response in citrinin (Gr I) was more compared to endosulfan (Gr II) treated rats as at 72 hrs the ear pinna thickness in citrinin was more than endosulfan (0.76 ± 0.011 mm vs 0.68 ± 0.006 mm), and identical findings were seen in stimulation index by LTT (1.00 ± 0.001 vs 0.90 ± 0.001). In humoral immune response the trend changed, by hemagglutination the animals in group I (citrinin) showed less titre than group II (endosulfan) (2.83 ± 0.31 vs 4.00 ± 0.26), however, by indirect ELISA the titre of citrinin (gr I) was more than that of endosulfan (gr II) (0.081 ± 0.003 vs 0.073 ± 0.001). However, in the combined group the animals were significantly more immunocompromised when adjudged for cell mediated (DTH: 0.57 ± 0.008 mm; LTT: 0.80 ± 0.007) and humoral immune responses (HA: 2.33 ± 0.21; indirect ELISA: 0.066 ± 0.001) as compared to the individually intoxicated groups and control (DTH: 0.94 ±0.012 mm; LTT: 1.10 ± 0.001), (HA: 5.50 ± 0.22; indirect ELISA: 0.108 ± 0.006). It may be concluded that with simultaneous exposure CIT and endosulfan, are potent immunosuppressants, but when they were given together their effect was enhanced possibly due to their additional interaction in pregnant Wistar rats.

Ključne riječi

citrinin; endosulfan; humoral immune response; cell mediated immune response

Hrčak ID:

74667

URI

https://hrcak.srce.hr/74667

Datum izdavanja:

5.12.2011.

Podaci na drugim jezicima: hrvatski

Posjeta: 1.372 *