Izvorni znanstveni članak

https://doi.org/10.5562/cca1761

Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection

Laurian Vlase ; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Haţieganu”, Victor Babeş street 8, RO-400012, Cluj-Napoca, Romania
Silvia Imre ; Department of Drug Analysis, Faculty of Pharmacy, University of Medicine and Pharmacy, Gheorghe Marinescu street 38, RO-540139, Târgu-Mureş, Romania
Dana Muntean ; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Haţieganu”, Victor Babeş street 8, RO-400012, Cluj-Napoca, Romania
Marcela Achim ; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Haţieganu”, Victor Babeş street 8, RO-400012, Cluj-Napoca, Romania
Daniela-Lucia Muntean ; Department of Drug Analysis, Faculty of Pharmacy, University of Medicine and Pharmacy, Gheorghe Marinescu street 38, RO-540139, Târgu-Mureş, Romania

Sažetak

A new simple, sensitive and LC-MS/MS method for quantification of spironolactone and its metabolite,
canrenone, in human plasma is proposed. The analytes were analysed on a C18 column at 48 ºC,
by using a mobile phase of 58 % methanol, 42 % 10 mmol dm−3 ammonim acetate in water and a flow
rate of 1 mL/min. The detection of both analytes in plasma was performed as follows: ESI+, EIC (m/z
169;187;283;305) from m/z 341, after protein precipitation with methanol. Calibration curves were generated
over the ranges 2.77–184.50 ng/mL for spironolactone and 2.69–179.20 ng/mL for canrenone by using
a weighted (1/y) linear regression. The absolute values of within- and between-run precision and accuracy
for both analytes ranged between 3.1 and 13.9 %, and the mean recovery was 99.7 %. The analytes
demonstrated good stability in various conditions. The validated method has been applied to a bioequivalence
study of 50 mg spironolactone tablets on healthy volunteers. (doi: 10.5562/cca1761)

Ključne riječi

spironolactone; canrenone; LC-MS/MS; pharmacokinetics; plasma

Hrčak ID:

74773

URI

https://hrcak.srce.hr/74773

Datum izdavanja:

13.11.2011.

Posjeta: 4.168 *