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https://doi.org/10.5562/cca1761

Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection

Laurian Vlase ; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Haţieganu”, Victor Babeş street 8, RO-400012, Cluj-Napoca, Romania
Silvia Imre ; Department of Drug Analysis, Faculty of Pharmacy, University of Medicine and Pharmacy, Gheorghe Marinescu street 38, RO-540139, Târgu-Mureş, Romania
Dana Muntean ; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Haţieganu”, Victor Babeş street 8, RO-400012, Cluj-Napoca, Romania
Marcela Achim ; Department of Pharmaceutical Technology and Biopharmaceutics, Faculty of Pharmacy, University of Medicine and Pharmacy “Iuliu Haţieganu”, Victor Babeş street 8, RO-400012, Cluj-Napoca, Romania
Daniela-Lucia Muntean ; Department of Drug Analysis, Faculty of Pharmacy, University of Medicine and Pharmacy, Gheorghe Marinescu street 38, RO-540139, Târgu-Mureş, Romania

Puni tekst: engleski, pdf (3 MB) str. 361-366 preuzimanja: 2.379* citiraj
APA 6th Edition
Vlase, L., Imre, S., Muntean, D., Achim, M. i Muntean, D. (2011). Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection. Croatica Chemica Acta, 84 (3), 361-366. https://doi.org/10.5562/cca1761
MLA 8th Edition
Vlase, Laurian, et al. "Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection." Croatica Chemica Acta, vol. 84, br. 3, 2011, str. 361-366. https://doi.org/10.5562/cca1761. Citirano 28.02.2021.
Chicago 17th Edition
Vlase, Laurian, Silvia Imre, Dana Muntean, Marcela Achim i Daniela-Lucia Muntean. "Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection." Croatica Chemica Acta 84, br. 3 (2011): 361-366. https://doi.org/10.5562/cca1761
Harvard
Vlase, L., et al. (2011). 'Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection', Croatica Chemica Acta, 84(3), str. 361-366. https://doi.org/10.5562/cca1761
Vancouver
Vlase L, Imre S, Muntean D, Achim M, Muntean D. Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection. Croatica Chemica Acta [Internet]. 2011 [pristupljeno 28.02.2021.];84(3):361-366. https://doi.org/10.5562/cca1761
IEEE
L. Vlase, S. Imre, D. Muntean, M. Achim i D. Muntean, "Determination of Spironolactone and Canrenone in Human Plasma by High-performance Liquid Chromatography with Mass Spectrometry Detection", Croatica Chemica Acta, vol.84, br. 3, str. 361-366, 2011. [Online]. https://doi.org/10.5562/cca1761

Sažetak
A new simple, sensitive and LC-MS/MS method for quantification of spironolactone and its metabolite,
canrenone, in human plasma is proposed. The analytes were analysed on a C18 column at 48 ºC,
by using a mobile phase of 58 % methanol, 42 % 10 mmol dm−3 ammonim acetate in water and a flow
rate of 1 mL/min. The detection of both analytes in plasma was performed as follows: ESI+, EIC (m/z
169;187;283;305) from m/z 341, after protein precipitation with methanol. Calibration curves were generated
over the ranges 2.77–184.50 ng/mL for spironolactone and 2.69–179.20 ng/mL for canrenone by using
a weighted (1/y) linear regression. The absolute values of within- and between-run precision and accuracy
for both analytes ranged between 3.1 and 13.9 %, and the mean recovery was 99.7 %. The analytes
demonstrated good stability in various conditions. The validated method has been applied to a bioequivalence
study of 50 mg spironolactone tablets on healthy volunteers. (doi: 10.5562/cca1761)

Ključne riječi
spironolactone; canrenone; LC-MS/MS; pharmacokinetics; plasma

Hrčak ID: 74773

URI
https://hrcak.srce.hr/74773

Posjeta: 2.794 *