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Izvorni znanstveni članak
https://doi.org/10.5562/cca1795
Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS
Annamária Bui
; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs
Anikó Kilár
; Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary; Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs,
Ágnes Dörnyei
; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs; Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs, Hungary
Viktória Poór
; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs
Krisztina Kovács
; Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary
Béla Kocsis
; Department of Medical Microbiology and Immunology, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs, Hungary
Ferenc Kilár
orcid.org/0000-0001-5737-5514
; Institute of Bioanalysis, Faculty of Medicine, University of Pécs, Szigeti út 12., H-7624 Pécs; Department of Analytical and Environmental Chemistry, Faculty of Sciences, University of Pécs, Ifjúság útja 6., H-7624 Pécs, Hungary
| Puni tekst: engleski, pdf (1 MB) | str. 393-398 | citiraj | |
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APA 6th Edition Bui, A., Kilár, A., Dörnyei, Á., Poór, V., Kovács, K., Kocsis, B. i Kilár, F. (2011). Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS. Croatica Chemica Acta, 84 (3), 393-398. https://doi.org/10.5562/cca1795 MLA 8th Edition Bui, Annamária, et al. "Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS." Croatica Chemica Acta, vol. 84, br. 3, 2011, str. 393-398. https://doi.org/10.5562/cca1795. Citirano 08.03.2021. Chicago 17th Edition Bui, Annamária, Anikó Kilár, Ágnes Dörnyei, Viktória Poór, Krisztina Kovács, Béla Kocsis i Ferenc Kilár. "Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS." Croatica Chemica Acta 84, br. 3 (2011): 393-398. https://doi.org/10.5562/cca1795 Harvard Bui, A., et al. (2011). 'Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS', Croatica Chemica Acta, 84(3), str. 393-398. https://doi.org/10.5562/cca1795 Vancouver Bui A, Kilár A, Dörnyei Á, Poór V, Kovács K, Kocsis B i sur. Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS. Croatica Chemica Acta [Internet]. 2011 [pristupljeno 08.03.2021.];84(3):393-398. https://doi.org/10.5562/cca1795 IEEE A. Bui, et al., "Carbohydrate Composition of Endotoxins from R-type Isogenic Mutants of Shigella sonnei Studied by Capillary Electrophoresis and GC-MS", Croatica Chemica Acta, vol.84, br. 3, str. 393-398, 2011. [Online]. https://doi.org/10.5562/cca1795 |
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Sažetak
The carbohydrate composition of the rough-type endotoxins (lipopolysaccharides, LPSs) from
Shigella sonnei mutant strains (Shigella sonnei phase II - 4303, 562H, R41 and 4350) was investigated by
capillary electrophoresis and GC-MS. The monosaccharides obtained by hydrolysis were determined by
capillary electrophoresis combined with laser induced fluorescence detection (CE-LIF) after labeling with
8-aminopyrene-1,3,6-trisulfonic acid (APTS) and by GC-MS as alditol-acetate derivatives. It was obtained
that the lipopolysaccharides of the isogenic rough mutants are formed in a step-like manner, containing
no heptose (4350), one D-glycero-D-mannoheptose (562H), or two or three L-glycero-Dmannoheptoses
(R41, 4303, respectively) in the deep core region. Besides the heptoses, the longest
LPS from the mutant Shigella sonnei 4303 contains hexoses, such as glucoses and galactoses, in a proportion
of approximately 3:2. This study provides a comprehensive comparison of the variability in
the carbohydrate part of the rough endotoxins extracted from Shigella sonnei isogenic mutants.
(doi: 10.5562/cca1795)
Ključne riječi
lipopolysaccharide structure; Shigella sonnei rough mutant; D-glycero-D-mannoheptose; capillary electrophoresis; CE-LIF; GC-MS
Hrčak ID: 74778
URI
https://hrcak.srce.hr/74778
Moj profil
Srce