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https://doi.org/10.5562/cca1788

HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds

Augustin Curticapean ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Felicia Toma ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Monica Tarcea ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Manuela Curticapean ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Victor Samarghitan ; University of Medicine and Pharmacy, 38 Gh. Marinescu Street, RO-540139 Târgu-Mures, Romania
Ioan Aurel Pop ; S.C.D.C.B. Tg-Mures, Str. Principala 1227, RO-547530 Sangeorgiu de Mures, Romania
Alin Gulea ; S.C.D.C.B. Tg-Mures, Str. Principala 1227, RO-547530 Sangeorgiu de Mures, Romania

Puni tekst: engleski, pdf (2 MB) str. 413-417 preuzimanja: 5.840* citiraj
APA 6th Edition
Curticapean, A., Toma, F., Tarcea, M., Curticapean, M., Samarghitan, V., Pop, I.A. i Gulea, A. (2011). HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds. Croatica Chemica Acta, 84 (3), 413-417. https://doi.org/10.5562/cca1788
MLA 8th Edition
Curticapean, Augustin, et al. "HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds." Croatica Chemica Acta, vol. 84, br. 3, 2011, str. 413-417. https://doi.org/10.5562/cca1788. Citirano 05.03.2021.
Chicago 17th Edition
Curticapean, Augustin, Felicia Toma, Monica Tarcea, Manuela Curticapean, Victor Samarghitan, Ioan Aurel Pop i Alin Gulea. "HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds." Croatica Chemica Acta 84, br. 3 (2011): 413-417. https://doi.org/10.5562/cca1788
Harvard
Curticapean, A., et al. (2011). 'HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds', Croatica Chemica Acta, 84(3), str. 413-417. https://doi.org/10.5562/cca1788
Vancouver
Curticapean A, Toma F, Tarcea M, Curticapean M, Samarghitan V, Pop IA i sur. HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds. Croatica Chemica Acta [Internet]. 2011 [pristupljeno 05.03.2021.];84(3):413-417. https://doi.org/10.5562/cca1788
IEEE
A. Curticapean, et al., "HPLC Method Validation for Simultaneous Determination of Three Mycotoxins from Corn Seeds", Croatica Chemica Acta, vol.84, br. 3, str. 413-417, 2011. [Online]. https://doi.org/10.5562/cca1788

Sažetak
A new HPLC optimized method for simultaneous determination of some mycotoxins by separation
and using a Dionex UltiMate3000 modular system, with multichannel UV detector was developed
and validated. The method optimization was performed to determine simultaneously, several relevant mycotoxins
from corn seeds that were stored over 8 months. The mycotoxins selected for quantification
were: aflatoxin B1, ochratoxin A and zearalenone. They were selected due to their high concentration in
the fodder. For the development stages of the HPLC method, it was introduced an internal standard to
have accurate results. Taking into account that the analytes should be extracted from the complex matrix
they reside, an extraction procedure was performed, using organic solvents, and the selection after
repeated tests demonstrated the best capacity for ethyl acetate. The recovery is about 60–70 % after the
extraction process also there is a good preconcentration (×2.5) of the analytes and the internal standard for
their quantification.
The limit of quantification (LOQ) obtained by chromatographic parameters optimization, for aflatoxin
B1 and ochratoxin A were about 3–5 μg kg–1 and 14.4 μg kg–1 for zearalenone of raw biological
material, making these values lower than those accepted by the actual normatives and regulations.
(doi: 10.5562/cca1788)

Ključne riječi
method validation; mycotoxins; corn seeds analysis

Hrčak ID: 74806

URI
https://hrcak.srce.hr/74806

Posjeta: 6.237 *