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A Tetrahydrophthalazine Derivative »Sodium Nucleinate« Exerts a Potent Suppressive Effect upon LPS-Stimulated Mononuclear Cells in vitro and in vivo
Tomislav Jukić
Musa Abidov
Alojz Ihan
Sažetak
We described the use of a new chemical substance Sodium nucleinate (SN) as an immunomodulatory substance exhibiting
antiinflammatory properties. Sodium nucleinate (SN) registrated in Russian Federation as Tamerit®, is 2-amino-
1,2,3,4-tetrahydrophthalazine-1,4-dione sodium salt dihydrate, derivative of well known chemical substance luminol.
To comprehend the mechanisms of SN immunomodulatory activity, we examined the SN modulation of the innate
inflammatory cytokine response of human PBMC stimulated with LPS in vitro. Furthermore, we studied the immunomodulatory
effects of SN in mice challenged with E. coli LPS in vivo to investigate a possible novel approach to therapy
of excessive inflammation that interfere with the response to endotoxin and inflammatory mediators. Our results demonstrated
that SN is an efficient inhibitor of sepsis development in mice model of LPS-induced sepsis. The changes induced by
SN include decreased mice plasma inflammatory cytokine production. Simmilary we demonstrated a decreased TNF-a,
IFN-g and IL-6 response in human LPS-stimulated PBMNCs. SN was therefore shown to be a promising inhibitor of
multiple inflammatory cytokine secretion.
Ključne riječi
sepsis; lipopolysaccharide; tumor necrosis factor alpha; gamma interferon; interleukin 6; interleukin 12
Hrčak ID:
75642
URI
Datum izdavanja:
30.12.2011.
Posjeta: 914 *