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Chemical and Biochemical Engineering Quarterly, Vol. 25 No. 4, 2011.

Izvorni znanstveni članak

Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli

D. Stanzer ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
J. Mrvčić ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
S. Križanović ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
V. Stehlik-Tomas ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia
S. Grba ; Faculty of Food Technology and Biotechnology, University of Zagreb, Pierottijeva 6, HR-10000 Zagreb, Croatia

Puni tekst: engleski, pdf (137 KB) str. 513-517 preuzimanja: 147* citiraj
APA 6th Edition
Stanzer, D., Mrvčić, J., Križanović, S., Stehlik-Tomas, V. i Grba, S. (2011). Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli. Chemical and Biochemical Engineering Quarterly, 25 (4), 513-517. Preuzeto s https://hrcak.srce.hr/76444
MLA 8th Edition
Stanzer, D., et al. "Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli." Chemical and Biochemical Engineering Quarterly, vol. 25, br. 4, 2011, str. 513-517. https://hrcak.srce.hr/76444. Citirano 21.05.2019.
Chicago 17th Edition
Stanzer, D., J. Mrvčić, S. Križanović, V. Stehlik-Tomas i S. Grba. "Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli." Chemical and Biochemical Engineering Quarterly 25, br. 4 (2011): 513-517. https://hrcak.srce.hr/76444
Harvard
Stanzer, D., et al. (2011). 'Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli', Chemical and Biochemical Engineering Quarterly, 25(4), str. 513-517. Preuzeto s: https://hrcak.srce.hr/76444 (Datum pristupa: 21.05.2019.)
Vancouver
Stanzer D, Mrvčić J, Križanović S, Stehlik-Tomas V, Grba S. Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli. Chemical and Biochemical Engineering Quarterly [Internet]. 2011 [pristupljeno 21.05.2019.];25(4):513-517. Dostupno na: https://hrcak.srce.hr/76444
IEEE
D. Stanzer, J. Mrvčić, S. Križanović, V. Stehlik-Tomas i S. Grba, "Enhancement of Trigonopsis variabilis D-Amino Acid Oxidase Overproduction in Fed-batch Cultivation of Escherichia coli", Chemical and Biochemical Engineering Quarterly, vol.25, br. 4, str. 513-517, 2011. [Online]. Dostupno na: https://hrcak.srce.hr/76444. [Citirano: 21.05.2019.]

Sažetak
D-Amino acid oxidase is a key enzyme in the production of semisynthetic cephalosporins. The Trigonopsis variabilis D-amino acid oxidase with 12- amino-acid peptide at N-terminus was expressed in Escherichia coli BL21(DE3), in a series of short fed-batch cultivations. The strategy of gradual induction and gradual feeding with D-methionine during expression resulted in active enzyme production twice as high as that in which the inducer and methionine were added all at once at the expression start. In optimal conditions during 14 h of aerobic cultivation, 350 mg L–1 of active enzyme was produced.

Ključne riječi
D-Amino acid oxidase; Trigonopsis variabilis; Escherichia coli; D-methionine

Hrčak ID: 76444

URI
https://hrcak.srce.hr/76444

Posjeta: 324 *