Stručni rad
Effects of sodium hypochlorite /NaCIO/ and mercuric (II) chlorite /HgCI2/ on surface sterilization of Oblačinska sour cherry buds in in vitro culture
Ines Mihaljević
; Poljoprivredni institut Osijek, Osijek, Hrvatska
Vesna Tomaš
; Poljoprivredni institut Osijek, Osijek, Hrvatska
Krunoslav Dugalić
; Poljoprivredni institut Osijek, Osijek, Hrvatska
Zorica Jurković
; Poljoprivredni institut Osijek, Osijek, Hrvatska
Marija Viljevac
; Poljoprivredni institut Osijek, Osijek, Hrvatska
Boris Puškar
Ankica Pranjić
Sažetak
To obtain in vitro culture it is very important to find a solvent for removing bacterial and fungi from explant tissue. Surface sterilization is the most important step in preparation of explants. In the present study research was conducted on sour cherry cv. Oblačinska višnja, clone OS. A comparison was made between sodium hypochlorite (NaOCl) and mercuric chloride (HgCl₂) with different times of buds sterilization. First, buds were sterilized with 70% of ethanol for few seconds and after that they were sterilized with sodium hypochlorite (concentration 1, 2, and 3%, for 20, 15 and 10 min.) and mercuric chlorite (concentration 0.1, 0.5 and 1%, for 10, 5 and 2 min.) Sterilized explants were inoculated on culture medium. After one month the number of contaminated, collapsed and healthy plants was determinate. The best results were obtained by surface sterilization with 1% NaOCl with exposure of 20 min, with 80% healthy, survival plants.
Ključne riječi
contamination; explant; in vitro plant; Oblačinska višnja; sterilization
Hrčak ID:
77454
URI
Datum izdavanja:
9.2.2012.
Posjeta: 1.516 *