Izvorni znanstveni članak
Genotyping of Salmonella enterica serovar Typhi strains isolated from 1959 to 2006 in China and analysis of genetic diversity by genomic microarray
Haifang Zhang
; Department of Biochemistry and Molecular Biology, School of Basic Medicine and Medical Technology Jiangsu University, Zhenjiang, Jiangsu, China
Xiaolei Zhang
; Department of Biochemistry and Molecular Biology, School of Basic Medicine and Medical Technology Jiangsu University, Zhenjiang, Jiangsu, China
Meiying Yan
; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
Bo Pang
; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
Biao Kan
; State Key Laboratory for Infectious Disease Prevention and Control, National Institute for Communicable Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Beijing, China
Huaxi Xu
; Department of Biochemistry and Molecular Biology, School of Basic Medicine and Medical Technology Jiangsu University, Zhenjiang, Jiangsu, China
Xinxiang Huang
; Department of Biochemistry and Molecular Biology, School of Basic Medicine and Medical Technology Jiangsu University, Zhenjiang, Jiangsu, China
Sažetak
Aim To determine the genotype of Salmonella enterica serovar
Typhi (S. Typhi) strains in China and analyze their genetic
diversity.
Methods We collected S. Typhi strains from 1959 to 2006
in five highly endemic Chinese provinces and chose 40
representative strains. Multilocus sequence typing was
used to determine the genotypes or sequence types (ST)
and microarray-based comparative genomic hybridization
(M-CGH) to investigate the differences in gene content
among these strains.
Results Forty representative S. Typhi strains belonged to 4
sequence types (ST1, ST2, ST890, and ST892). The predominant
S. Typhi genotype (31/40) was ST2 and it had a diverse
geographic distribution. We discovered two novel STs –
ST890 and ST892. M-CGH showed that 69 genes in these
two novel STs were divergent from S. Typhi Ty2, which belongs
to ST1. In addition, 5 representative Typhi strains of
ST2 isolated from Guizhou province showed differences in
divergent genes.
Conclusion We determined two novel sequence types,
ST890 and ST892, and found that ST2 was the most prevalent
genotype of S. Typhi in China. Genetic diversity was
present even within a highly clonal bacterial population.
Ključne riječi
Salmonella enterica serovar Typhi; genotype; multi-locus sequence typing; novel sequence type; microarray-based comparative genomic hybridization; genetic diversity
Hrčak ID:
78198
URI
Datum izdavanja:
15.12.2011.
Posjeta: 1.007 *