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https://doi.org/10.2478/10004-1254-63-2012-2155

Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran

Ali Reza Khosravi ; Mycology Research Center, Faculty of Veterinary Medicine, Tehran, Iran
Mahnaz Fatahinia ; Mycology Research Center, Faculty of Veterinary Medicine, Tehran, Iran
Hojjatollah Shokri ; Faculty of Veterinary, Iran
Mohammad Hossein Yadegari ; Department of Mycology, Faculty of Medicine, University of Tarbiat Modares, Tehran, Iran

Puni tekst: engleski, pdf (104 KB) str. 1-5 preuzimanja: 460* citiraj
APA 6th Edition
Khosravi, A.R., Fatahinia, M., Shokri, H. i Yadegari, M.H. (2012). Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran. Arhiv za higijenu rada i toksikologiju, 63 (1), 1-5. https://doi.org/10.2478/10004-1254-63-2012-2155
MLA 8th Edition
Khosravi, Ali Reza, et al. "Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran." Arhiv za higijenu rada i toksikologiju, vol. 63, br. 1, 2012, str. 1-5. https://doi.org/10.2478/10004-1254-63-2012-2155. Citirano 23.09.2020.
Chicago 17th Edition
Khosravi, Ali Reza, Mahnaz Fatahinia, Hojjatollah Shokri i Mohammad Hossein Yadegari. "Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran." Arhiv za higijenu rada i toksikologiju 63, br. 1 (2012): 1-5. https://doi.org/10.2478/10004-1254-63-2012-2155
Harvard
Khosravi, A.R., et al. (2012). 'Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran', Arhiv za higijenu rada i toksikologiju, 63(1), str. 1-5. https://doi.org/10.2478/10004-1254-63-2012-2155
Vancouver
Khosravi AR, Fatahinia M, Shokri H, Yadegari MH. Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran. Arh Hig Rada Toksikol. [Internet]. 2012 [pristupljeno 23.09.2020.];63(1):1-5. https://doi.org/10.2478/10004-1254-63-2012-2155
IEEE
A.R. Khosravi, M. Fatahinia, H. Shokri i M.H. Yadegari, "Allergens from Fusarium solani Identified by Immunoblotting in Asthma Patients in Iran", Arhiv za higijenu rada i toksikologiju, vol.63, br. 1, str. 1-5, 2012. [Online]. https://doi.org/10.2478/10004-1254-63-2012-2155

Sažetak
We extracted Fusarium solani antigens to evaluate specifi c anti-F. solani IgE in fifty-one patients with asthma (33 men and 18 women) and in 22 non-atopic healthy subjects (15 men and 7 women). F. solani strains were cultured in Sabouraud glucose agar and subjected to cell disruption using the freeze-and-thaw method. The obtained cytoplasmic extracts were analysed using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Sensitisation to F. solani antigens has been evaluated in asthmatic patients using the immunoblotting assay. The SDS-PAGE identifi ed 29 protein bands in the cytoplasmic extracts of F. solani isolates, with molecular weights ranging from 24 kDa to 112 kDa. Immunoblotting detected specific anti-F. solani IgE antibody in all asthma patients, but not in the control group. The predominant reactive allergens in patients corresponded to the bands with molecular weights of 24 kDa, 58.5 kDa, 64.5 kDa, 69 kDa, 72 kDa, and 97 kDa. Our results suggest that various allergenic components of F. solani may produce symptoms of asthma in susceptible individuals and they call for further research.

Ključne riječi
airborne spores; allergenic sensitisation; fungal allergy; IgE; SDS-PAGE

Hrčak ID: 78630

URI
https://hrcak.srce.hr/78630

[hrvatski]

Posjeta: 833 *