hrcak mascot   Srce   HID

Review article

Microorganisms in stable air as possible postsecretory milk contaminants

Kristina Matković ; Veterinarski fakultet Sveučilišta u Zagrebu, Zavod za animalnu higijenu, okoliš i etologiju , Heinzelova 55, 10000 Zagreb
Marija Vučemilo
Bara Vinković
Željko Pavičić
Srećko Matković

Fulltext: croatian, pdf (217 KB) pages 369-377 downloads: 1.241* cite
APA 6th Edition
Matković, K., Vučemilo, M., Vinković, B., Pavičić, Ž. & Matković, S. (2006). Mikroorganizmi u zraku staje kao mogući postsekretorni zagađivači mlijeka. Mljekarstvo, 56 (4), 369-377. Retrieved from
MLA 8th Edition
Matković, Kristina, et al. "Mikroorganizmi u zraku staje kao mogući postsekretorni zagađivači mlijeka." Mljekarstvo, vol. 56, no. 4, 2006, pp. 369-377. Accessed 30 Jul. 2021.
Chicago 17th Edition
Matković, Kristina, Marija Vučemilo, Bara Vinković, Željko Pavičić and Srećko Matković. "Mikroorganizmi u zraku staje kao mogući postsekretorni zagađivači mlijeka." Mljekarstvo 56, no. 4 (2006): 369-377.
Matković, K., et al. (2006). 'Mikroorganizmi u zraku staje kao mogući postsekretorni zagađivači mlijeka', Mljekarstvo, 56(4), pp. 369-377. Available at: (Accessed 30 July 2021)
Matković K, Vučemilo M, Vinković B, Pavičić Ž, Matković S. Mikroorganizmi u zraku staje kao mogući postsekretorni zagađivači mlijeka. Mljekarstvo [Internet]. 2006 [cited 2021 July 30];56(4):369-377. Available from:
K. Matković, M. Vučemilo, B. Vinković, Ž. Pavičić and S. Matković, "Mikroorganizmi u zraku staje kao mogući postsekretorni zagađivači mlijeka", Mljekarstvo, vol.56, no. 4, pp. 369-377, 2006. [Online]. Available: [Accessed: 30 July 2021]

Quality and hygienic correct milk must satisfy conditions which are prescribed by Law on Raw Milk Quality (NN102/2000). If we know that freshly milked milk could contain a certain number of microorganisms originating from the stable air (so called postsecretory contamination), performed research can help in establishing microclimatic factors that can influence that kind of contamination. Research was carried out in a dairy stable, basic microclimatic parameters were analyzed as well as number of microorganisms in the stable air. Measurements were executed in the morning, at noon and in the evening, once a week, during two months. Microclimate parameters were determined by standard, attested Testo 400 device (Testo Inc., Germany) and Merck MAS-100 device (Merck KgaA, Darmstadt, Germany). Air samples were inoculated on prepared Columbia and Sabouraud agar (Biolife, Milan, Italy). After incubation and counting, the most common colonies were reinoculated on selective medium and identified by microbiological procedures. Obtained values were analyzed by statistical program Statistica. Mean values of total bacterial count in stable air were between 8.81 x 104 CFU/m3 at noon up to 1.26 x 105 CFU/m3 in the evening, and mean values of total moulds count in stable air were between 5.23 x 104 CFU/m3 at noon up to 8.35 x 104 CFU/m3 in the morning. Ten bacterial genus’s were identified. The most common bacteria in stable air were gram-positive bacteria, specially Staphylococcus i Streptococcus genera. Nine mould genera were isolated, predominated by the genera Aspergillus, Penicillium and yeasts. By Wilcoxon matched pair test at a level of statistical significance of p<0.05 was demonstrated that air temperature, relative humidity and air flow velocity directly influence on total number of microorganisms in stable air. Number and species of identified microorganisms in stable air could have influence on hygiene quality of fresh milk. Therefore, by regularly conduction of zoohygienic measures, regulatory udder hygiene before and after milking as well as milking equipment hygiene have to be conducted, to prevent postsecretory milk contamination.

microorganisms; air; milk; pollution

Hrčak ID: 8413



Visits: 2.196 *