Izvorni znanstveni članak
https://doi.org/10.2478/10004-1254-63-2012-2218
Comparison of Buffers for Extraction of Mite Allergen Der p 1 from Dust
Ljerka Prester
; Institute for Medical Research and Occupational Health Zagreb, Croatia
Jelena Kovačić
; Institute for Medical Research and Occupational Health Zagreb, Croatia
Jelena Macan
; Institute for Medical Research and Occupational Health Zagreb, Croatia
Sažetak
Der p 1 is the main allergen of house dust mite Dermatophagoides pteronyssinus, which has routinely been detected in residential dust. However, the procedure for extracting Der p 1 from reservoir dust has not been well defined. The aim of this study was to compare Der p 1 mass fractions in dust extracts prepared using the following extraction buffers: phosphate (pH 7.4), borate (pH 8.0), and ammonium bicarbonate (pH 8.0), all with 0.05 % Tween 20. Twenty-eight dust samples were divided into three aliquots and each portion was extracted with one of the three buffers at room temperature. Der p 1 mass fractions were measured in a total of 84 dust extracts using the enzyme immunoassay (range: 0.1 μg g-1 to 7.53 μg g-1). Statistical methods including intraclass correlation showed a high agreement between Der p 1 mass fractions irrespective of the extracting medium. Our results suggest that all three buffers are suitable for the extraction of mite allergens and routine Der p 1 analysis in dust.
Ključne riječi
Dermatophagoides pteronyssinus; ELISA; extraction buffers; indoor allergens; intraclass correlation; settled dust
Hrčak ID:
86887
URI
Datum izdavanja:
25.9.2012.
Posjeta: 2.446 *