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Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues

Lejla Duraković ; University of Zagreb, Faculty of Food Technology and Biotechnology, Department of Biochemical Engineering, Laboratory for General Microbiology and Food Microbiology, Pierottijeva 6, 10000 Zagreb, Croatia
Frane Delaš
Alma Tudić
Katarina Huić-Babić
Sulejman Redžepović

Puni tekst: engleski, pdf (295 KB) str. 179-191 preuzimanja: 596* citiraj
APA 6th Edition
Duraković, L., Delaš, F., Tudić, A., Huić-Babić, K. i Redžepović, S. (2012). Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues. Mljekarstvo, 62 (3), 179-191. Preuzeto s https://hrcak.srce.hr/86914
MLA 8th Edition
Duraković, Lejla, et al. "Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues." Mljekarstvo, vol. 62, br. 3, 2012, str. 179-191. https://hrcak.srce.hr/86914. Citirano 17.09.2019.
Chicago 17th Edition
Duraković, Lejla, Frane Delaš, Alma Tudić, Katarina Huić-Babić i Sulejman Redžepović. "Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues." Mljekarstvo 62, br. 3 (2012): 179-191. https://hrcak.srce.hr/86914
Harvard
Duraković, L., et al. (2012). 'Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues', Mljekarstvo, 62(3), str. 179-191. Preuzeto s: https://hrcak.srce.hr/86914 (Datum pristupa: 17.09.2019.)
Vancouver
Duraković L, Delaš F, Tudić A, Huić-Babić K, Redžepović S. Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues. Mljekarstvo [Internet]. 2012 [pristupljeno 17.09.2019.];62(3):179-191. Dostupno na: https://hrcak.srce.hr/86914
IEEE
L. Duraković, F. Delaš, A. Tudić, K. Huić-Babić i S. Redžepović, "Removal of aflatoxin M1 from artificially contaminated yoghurt by using of new synthesized dehydroacetic acid analogues", Mljekarstvo, vol.62, br. 3, str. 179-191, 2012. [Online]. Dostupno na: https://hrcak.srce.hr/86914. [Citirano: 17.09.2019.]

Sažetak
Dehydroacetic acid (DHA) and its new synthesized analogues, 4-hydroxy-3-(p-toluoyl)-6-(ptolyl)-2H-pyrane-2-one (DHT) and 5-Bromo-4-hydroxy-3-(p-toluoyl)-6-(p-tolyl)-2H-pyrane-2-one (BrDHT) were tested for removal of aflatoxin M1 (AFM1) from artificially contaminated yoghurt with known concentrations of this toxin to determine the possible use of these chemicals as a means of controlling AFM1 accumulation. Yoghurt from cow’s milk was artificially contaminated with AFM1 at levels of 0.01 to 0.5 μg/L. Yoghurts were stored at 4 °C and 7 °C, respectively, for up to 28 days. Analysis of AFM1 in yoghurt was carried out using two dimensional thin-layer chromatography (TLC) - visual estimation. The limit of detection was 0.15 ng/L. The recoveries of AFM1 from the samples spiked at levels of 10, 50, 100, and 500 ng/L were between 80.6 and 107.8 %, respectively. Concentrations of DHA and DHT of 0.01 and 0.03 μmol/L had non or little effect on AFM1 content in experimentally contaminated yoghurt, whereas concentrations higher than 0.05 μmol/L, partially inhibited AFM1 content. The percentage loss of the initial AFM1 amount in yoghurt was estimated by about 15 and 25 %, and 22 to 45 % by the end of storage, respectively. In experiments with 0.01 and 0.05 μmol/L of BrDHT or higher, the concentration of AFM1 was reduced after 28 days by 20 to 95 % or completely, respectively, depending on the time and temperature of deposit. Detection of toxicity of investigated analogues was evaluated by using the brine shrimp (Artemia salina) larvae as a screening system for the determination of their sensitivity to some chemicals.

Ključne riječi
aflatoxin M1; yoghurt; dehydroacetic acid analogues; TLC; Artemia salina

Hrčak ID: 86914

URI
https://hrcak.srce.hr/86914

[hrvatski]

Posjeta: 1.145 *