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Original scientific paper

Proizvodnja amilaza i proteaza iz industrijskog otpada pomoću bakterije Bacillus caldolyticus

Thorsten Jamrath ; Beuth Hochschule für Technik Berlin, University of Applied Sciences, FBV – Life Sciences and Technology, Seestrasse 64, DE-13347 Berlin, Germany
Christina Lindner ; Beuth Hochschule für Technik Berlin, University of Applied Sciences, FBV – Life Sciences and Technology, Seestrasse 64, DE-13347 Berlin, Germany
Milan K. Popović ; Beuth Hochschule für Technik Berlin, University of Applied Sciences, FBV – Life Sciences and Technology, Seestrasse 64, DE-13347 Berlin, Germany
Rakesh Bajpai ; University of Louisiana, Chemical Engineering Department, 131 Rex Street, 218 E Madison Hall, Lafayette, LA 70504, USA

Fulltext: english, pdf (250 KB) pages 355-361 downloads: 2.227* cite
APA 6th Edition
Jamrath, T., Lindner, C., Popović, M.K. & Bajpai, R. (2012). Production of Amylases and Proteases by Bacillus caldolyticus from Food Industry Wastes. Food Technology and Biotechnology, 50 (3), 355-361. Retrieved from https://hrcak.srce.hr/86930
MLA 8th Edition
Jamrath, Thorsten, et al. "Production of Amylases and Proteases by Bacillus caldolyticus from Food Industry Wastes." Food Technology and Biotechnology, vol. 50, no. 3, 2012, pp. 355-361. https://hrcak.srce.hr/86930. Accessed 3 Dec. 2021.
Chicago 17th Edition
Jamrath, Thorsten, Christina Lindner, Milan K. Popović and Rakesh Bajpai. "Production of Amylases and Proteases by Bacillus caldolyticus from Food Industry Wastes." Food Technology and Biotechnology 50, no. 3 (2012): 355-361. https://hrcak.srce.hr/86930
Harvard
Jamrath, T., et al. (2012). 'Production of Amylases and Proteases by Bacillus caldolyticus from Food Industry Wastes', Food Technology and Biotechnology, 50(3), pp. 355-361. Available at: https://hrcak.srce.hr/86930 (Accessed 03 December 2021)
Vancouver
Jamrath T, Lindner C, Popović MK, Bajpai R. Production of Amylases and Proteases by Bacillus caldolyticus from Food Industry Wastes. Food Technology and Biotechnology [Internet]. 2012 [cited 2021 December 03];50(3):355-361. Available from: https://hrcak.srce.hr/86930
IEEE
T. Jamrath, C. Lindner, M.K. Popović and R. Bajpai, "Production of Amylases and Proteases by Bacillus caldolyticus from Food Industry Wastes", Food Technology and Biotechnology, vol.50, no. 3, pp. 355-361, 2012. [Online]. Available: https://hrcak.srce.hr/86930. [Accessed: 03 December 2021]

Abstracts
Amilaze i proteaze se primijenjuju u industrijskim procesima, npr. za likvefakciju škroba ili kao dodaci detergentima. Enzimi koji sudjeluju u tim procesima moraju biti stabilni pri visokim temperaturama (>70 °C). U ovom je radu opisan postupak proizvodnje termostabilne ƒα-amilaze i neutralne proteaze pomoću termofilnog soja bakterije Bacillus caldolyticus DSM 405. Svrha je rada bila smanjiti cijenu proizvodnje zamjenom skupih sastojaka kontrolne podloge, kao što su nehidrolizirani škrob i pepton, jeftinim otpacima prehrambene industrije, poput nusproizvoda dobivanja škroba iz krumpira ili graška (sok i pulpa), sirutke, pivskog tropa, pulpe graška, ostataka kruha i svinjske krvi. Provedena su usporedna istraživanja u tikvicama na tresilici. Uporabom tih otpadaka znatno se poboljšala aktivnost ƒα-amilaze i smanjila cijena podloge. Primjenom pulpe graška povećala se aktivnost α-amilaze za 160 %, a smanjila cijena podloge za 97 % u usporedbi s kontrolnom podlogom. Troškovi podloge za proizvodnju proteaza također su smanjeni za više od 50 %.

Keywords
termostabilne amilaze; termostabilne neutralne proteaze; otpaci prehrambene industrije; Bacillus caldolyticus

Hrčak ID: 86930

URI
https://hrcak.srce.hr/86930

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