Radovi, Vol. 41 No. 1/2, 2006.
Izvorni znanstveni članak
COMPARISON OF MOLECULAR AND GENETIC PROPERTIES OF PINE (Pinus sylvestris L.) SEED PLANTATIONS IN BOSNIA AND HERCEGOVINA
D. Ballian
M. Konnert
F. Bogunić
Sažetak
The paper analyses the genetic structure of the pine (Pinus sylvestris L.) clones in the multiclone seed plantations of Kozji Grm (KG) and Šamin Gaj (ŠG). The clones in both seed plantations originate from natural populations of common pine in Bosnia and Hercegovina; KG (40 clones): Gornji Janj – 10 clones, Klekovača 7, Kaljina Bioštica 16, Romanija Glasinac 6, Igman 1, while the ŠG plantation contains 20 clones from one population (Igman). The genetic structure was analysed using 9 enzyme systems upon altogether 12 gene loci. Altogether 55 different alleles were observed: in KG plantation 45 alleles were seen, while in ŠG plantation there were 35 alleles. To estimate the genetic diversity, the mean number of alleles per locus (A/L) was calculated: it is 3.83 for KG, and 2.91 for ŠG, while the mean number of genotypes per locus (G/L) was 5.16 for KG and 3.58 for ŠG. The results confirm the assumption that the number of possible classes with genotypes is higher than with alleles. The differences are the most conspicuous if the differences are observed in the occurrence of the individual alleles and the allele frequencies.
The mean real hetero-zygote property of the clones in KG plantation is 0.2833, while the same value in ŠG is 0.3166. The mean theoretical hetero/zygote property of KG plantation is 0.3366, and of ŠG plantation it is 0.3491. The present differences show a deviation
of the real status from the status of genetic balance according to the Hardy/Weinberg law. The results show that the use of the seed produced in the multiclone seed plantation, which originates from the clones from different provenances, may increase the risk of introducing undesired alleles into the natural population. A more acceptable solution would therefore be the seed plantations from the clones of a single population, and the use of their seed for the regeneration of this population.
Ključne riječi
ommon pine (Pinus sylvestris L.); clone; plantation; iso-enzymes; identification
Hrčak ID:
8657
URI
Datum izdavanja:
23.12.2006.
Posjeta: 961 *